SNAP25 Antibody

Synaptosomal-associated protein 25, Mouse Anti Human
Cat. No.
BT30742
Source
Synonyms
Super-Protein, SUP, RIC4, SEC9, SNAP, RIC-4, SNAP25, SNAP-25, Synaptosomal-associated protein 25, Synaptosomal-associated 25 kDa protein, FLJ23079, bA416N4.2, dJ1068F16.2.
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Usage
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Description

Product Specs

Introduction
Synaptic vesicle membrane docking and fusion are mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix, and the protein encoded by this gene contributes the other two. Therefore, the SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters.
Formulation
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods, store at -20°C. Prevent freeze-thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
SNAP25 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. The recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. The recommended starting dilution is 1:2,000.
Synonyms
Super-Protein, SUP, RIC4, SEC9, SNAP, RIC-4, SNAP25, SNAP-25, Synaptosomal-associated protein 25, Synaptosomal-associated 25 kDa protein, FLJ23079, bA416N4.2, dJ1068F16.2.
Purification Method
SNAP25 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P4E11AT.
Immunogen
Anti-human SNAP25 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human SNAP25 amino acids 1-206 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Introduction

Synaptosomal-Associated Protein 25 (SNAP-25) is a crucial protein involved in the regulation of neurotransmitter release at synapses. It is encoded by the SNAP25 gene located on chromosome 20p12.2 in humans . This protein is a member of the soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex, which is essential for synaptic vesicle fusion and neurotransmitter release.

Structure and Function

SNAP-25 is a 25 kDa protein that plays a pivotal role in the synaptic function of specific neuronal systems. It is a component of the trans-SNARE complex, which is responsible for membrane fusion specificity and directly executes fusion by forming a tight complex that brings the vesicle and plasma membranes together . This process is critical for the release of neurotransmitters into the synaptic cleft, facilitating neuronal communication.

Clinical Significance

Mutations in the SNAP25 gene have been associated with various neurological disorders, including developmental and epileptic encephalopathies, attention deficit hyperactivity disorder (ADHD), schizophrenia, Alzheimer’s disease, bipolar disorder, and botulism . These associations highlight the importance of SNAP-25 in maintaining normal neurological function and its potential as a therapeutic target.

Mouse Anti Human SNAP-25 Antibodies

Mouse anti-human SNAP-25 antibodies are widely used in scientific research to study the expression and function of SNAP-25 in various biological samples. These antibodies are developed in mice and are designed to specifically target and bind to the human SNAP-25 protein. They are utilized in several applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, and Enzyme-Linked Immunosorbent Assay (ELISA) .

Applications in Research
  1. Western Blot: Used to detect and quantify SNAP-25 protein levels in different tissue samples.
  2. Immunocytochemistry: Allows visualization of SNAP-25 localization within cells.
  3. Immunohistochemistry: Used to study the distribution of SNAP-25 in tissue sections.
  4. Flow Cytometry: Enables the analysis of SNAP-25 expression on a cell-by-cell basis.
  5. ELISA: Used for the quantitative measurement of SNAP-25 in various samples .

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