SMAC/DIABLO Antibody
Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, Smac protein, Direct IAP-binding protein with low pI, DIABLO, SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049.
Description
Product Specs
The antibody is supplied at a concentration of 1mg/ml in a solution of PBS at pH 7.4, supplemented with 10% glycerol and 0.02% sodium azide.
Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, Smac protein, Direct IAP-binding protein with low pI, DIABLO, SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049.
Product Science Overview
SMAC/DIABLO (Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low pI) is a mitochondrial protein that plays a crucial role in the regulation of apoptosis. This protein is encoded by the DIABLO gene in humans and is known for its ability to bind and neutralize inhibitor of apoptosis proteins (IAPs), thereby promoting the activation of caspases and the apoptotic process .
SMAC/DIABLO is initially synthesized as a precursor protein with a mitochondrial targeting sequence (MTS) at its N-terminal. Upon import into the mitochondria, this sequence is cleaved off, resulting in the mature form of the protein. The mature SMAC/DIABLO protein is characterized by an N-terminal tetrapeptide sequence (Ala-Val-Pro-Ile) that is essential for its interaction with IAPs .
The primary function of SMAC/DIABLO is to promote apoptosis by binding to IAPs, such as XIAP (X-linked Inhibitor of Apoptosis Protein), and preventing them from inhibiting caspases. This interaction is crucial for the activation of caspases, which are the key executioners of apoptosis .
Apoptosis, or programmed cell death, is a vital process for maintaining cellular homeostasis and eliminating damaged or unwanted cells. SMAC/DIABLO is released from the mitochondria into the cytosol in response to apoptotic stimuli. Once in the cytosol, it binds to IAPs and neutralizes their inhibitory effect on caspases, thereby facilitating the apoptotic process .
Due to its pro-apoptotic function, SMAC/DIABLO is implicated in various types of cancer. The overexpression of IAPs in cancer cells can lead to resistance to apoptosis, contributing to tumor growth and survival. Therefore, SMAC/DIABLO and its mimetics are being explored as potential therapeutic agents to enhance the efficacy of cancer treatments by promoting apoptosis in cancer cells .
Mouse anti-human SMAC/DIABLO antibodies are monoclonal antibodies developed to specifically recognize and bind to the human SMAC/DIABLO protein. These antibodies are widely used in research to study the expression and function of SMAC/DIABLO in various biological contexts. They are also utilized in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to detect and quantify SMAC/DIABLO in different samples .
