SMAC/DIABLO Human
SMAC/DIABLO Human Recombinant
Cat. No.
BT17229
Source
Escherichia Coli.
Synonyms
Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, Smac protein, Direct IAP-binding protein with low pI, DIABLO, SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Smac/Diablo Human Recombinant fused to N-terminal T7-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22 kDa.
Product Specs
Introduction
Smac/Diablo, a proapoptotic protein, enhances caspase activation within the cytochrome c/Apaf-1/caspase-9 pathway. This is achieved through its interaction with inhibitor of apoptosis proteins (IAPs), effectively neutralizing their inhibitory effects. Localized within mitochondria, Smac/Diablo is released into the cytosol during apoptosis, where it regulates IAPs' caspase inhibition. Notably, Smac/DIABLO expression levels correlate with renal cell carcinoma outcomes. The protein's dimeric structure suggests a low probability of dissociation post-expression, minimizing the likelihood of functional loss. Furthermore, Survivin, Smac/DIABLO, and PKC-? play crucial roles in FGF-2-mediated apoptosis inhibition within human small cell lung cancer cells. Mitochondrial survivin interacts with Smac/DIABLO, delaying its release. Downregulated Smac protein expression contributes to ovarian carcinogenesis and chemoresistance. Smac/DIABLO actively participates in the apoptosis induction pathway within tumor cells. Regulation of SMAC protein involves XIAP and proteasomal degradation. Additionally, SMAC protein plays a role in leukemic cell apoptosis and is released from multiple myeloma cells undergoing stress-induced apoptosis.
Description
Recombinant human Smac/Diablo, fused with an N-terminal T7-Tag, is produced in E. coli. This yields a single, non-glycosylated polypeptide chain comprising 199 amino acids, with a molecular weight of 22 kDa.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The Smac/Diablo solution is prepared in a buffer containing 20mM Tris at a pH of 7.5.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It's crucial to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the product exceeds 95.0%, as determined by the following analytical methods: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Synonyms
Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, Smac protein, Direct IAP-binding protein with low pI, DIABLO, SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049.
Source
Escherichia Coli.
Amino Acid Sequence
MASMTGGQQM GRGSMAVPIA QKSEPHSLSS EALMRRAVSL VTDSTSTFLS QTTYALIEAI TEYTKAVYTL TSLYRQYTSL LGKMNSEEED EVWQVIIGAR AEMTSKHQEY LKLETTWMTA VGLSEMAAEA AYQTGADQAS ITARNHIQLV KLQVEEVHQL SRKAETKLAE AQIEELRQKT QEEGEERAES EQEAYLRED.
Product Science Overview
Discovery and Function
SMAC/DIABLO was first identified as a protein that promotes apoptosis by neutralizing the inhibitory effects of Inhibitor of Apoptosis Proteins (IAPs). IAPs are a family of proteins that prevent apoptosis by inhibiting caspases, which are the enzymes responsible for the execution of cell death. SMAC/DIABLO promotes caspase activation by binding to IAPs and preventing them from inhibiting caspases .
Mechanism of Action
Upon apoptotic stimuli, SMAC/DIABLO is released from the mitochondria into the cytosol. In the cytosol, it interacts with IAPs, such as XIAP (X-linked Inhibitor of Apoptosis Protein), and disrupts their ability to inhibit caspases. This interaction facilitates the activation of caspases, leading to the execution of apoptosis .
Recombinant SMAC/DIABLO
Applications in Research
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