SLURP1 Human Dimer

Secreted LY6/PLAUR Domain Containing 1 Dimer Human Recombinant
Cat. No.
BT16980
Source
Escherichia Coli.
Synonyms
Secreted LY6/PLAUR Domain Containing 1, Lymphocyte Antigen 6-Like Secreted, Anti-Neoplastic Urinary Protein, ARS(Component B)-81/S, ARS Component B, SLURP-1, ANUP, ARS, Secreted Ly6/UPAR Related Protein 1, LY6LS, ArsB, MDM, SLURP1.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

SLURP1 Human Recombinant produced in E.Coli is a non-glycosylated homodimer (Leu23-leu103) containing 2x89 amino acids including an 8 aa His tag at N-terminus. The total calculated molecular mass for each chain is 9.9kDa.

Product Specs

Introduction
As a member of the Ly6/uPAR family, Secreted LY6/PLAUR Domain Containing 1 (SLURP1) is unique due to its lack of a GPI-anchoring signal sequence. It is believed to possess antitumor activity and serves as a marker for late skin differentiation. Furthermore, SLURP1 plays a vital role in preserving the physiological and structural integrity of the skin's keratinocyte layers. Genetically, the SLURP1 gene shares its chromosomal region with several members of the Ly6/uPAR family of glycoprotein receptors. Notably, mutations in the SLURP1 gene have been linked to Mal de Meleda, a rare autosomal recessive skin disorder.
Description
Recombinant Human SLURP1, produced in E. coli, is a non-glycosylated homodimer with a structure encompassing residues Leu23 to Leu103. Each chain consists of 2x89 amino acids, including an 8 amino acid His tag located at the N-terminus. The calculated molecular mass for each chain is 9.9kDa.
Physical Appearance
White powder, lyophilized and filtered.
Formulation
The SLURP1 protein solution, at a concentration of 0.5mg/ml in 20mM Tris buffer with 50mM NaCl at pH 8.0, undergoes a 0.4μm filtration process before being lyophilized.
Solubility
To generate a working stock solution, it is advised to add deionized water to the lyophilized pellet, aiming for a concentration of approximately 0.5mg/ml. Allow sufficient time for the pellet to fully dissolve. Note that SLURP1 is not sterile. Prior to using in cell culture, ensure the product is filtered through a sterile filter with an appropriate pore size.
Stability
For long-term storage, keep the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize freeze-thaw cycles. Reconstituted protein remains stable at 4°C for a limited period, showing no significant changes for up to two weeks.
Purity
The purity of SLURP1 is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
Secreted LY6/PLAUR Domain Containing 1, Lymphocyte Antigen 6-Like Secreted, Anti-Neoplastic Urinary Protein, ARS(Component B)-81/S, ARS Component B, SLURP-1, ANUP, ARS, Secreted Ly6/UPAR Related Protein 1, LY6LS, ArsB, MDM, SLURP1.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHLK CYTCKEPMTS ASCRTITRCK PEDTACMTTL VTVEAEYPFN QSPVVTRSCS SSCVATDPDS IGAAHLIFCC FRDLCNSEL.

Product Science Overview

Introduction

The Secreted LY6/PLAUR Domain Containing 1 Dimer, also known as SLURP1, is a protein encoded by the SLURP1 gene. This protein is a member of the Ly6/uPAR family, which is characterized by a distinct disulfide bridge pattern that creates the three-finger Ly6/uPAR (LU) domain . The SLURP1 protein is secreted and lacks a glycosylphosphatidylinositol (GPI)-anchoring signal sequence .

Structure and Function

SLURP1 is known for its antitumor activity and is implicated in maintaining the physiological and structural integrity of the keratinocyte layers of the skin . It down-regulates keratinocyte proliferation in vitro and may act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity . Additionally, SLURP1 inhibits alpha-7-dependent nAChR currents in an allosteric manner . In T cells, it may be involved in the regulation of intracellular calcium signaling . The protein also seems to have an immunomodulatory function in the cornea .

Genetic and Evolutionary Aspects

The SLURP1 gene is located in a chromosomal region that includes several members of the Ly6/uPAR family of glycoprotein receptors . The genes encoding Ly6/uPAR family proteins are conserved across different species and are clustered in syntenic regions on human chromosomes 8, 19, 6, and 11 . The Ly6/uPAR family proteins share a common structure but have varying expression patterns and functions .

Clinical Significance

Mutations in the SLURP1 gene have been associated with Mal de Meleda, a rare autosomal recessive skin disorder . This disorder is characterized by palmoplantar keratoderma, which involves thickening of the skin on the palms and soles . The SLURP1 protein’s role in maintaining skin integrity highlights its importance in dermatological health.

Research and Applications

Research on SLURP1 has revealed its potential as a marker of late differentiation of the skin . Its antitumor properties and involvement in modulating nAChRs make it a significant target for further studies in cancer research and neurobiology . The recombinant form of SLURP1 is used in various experimental setups to study its functions and potential therapeutic applications.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

SLURP1 Human Monomer
Secreted LY6/PLAUR Domain Containing 1 Monomer Human Recombinant
Cat. No.
BT17146
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.