SLC51B Human

Solute Carrier Family 51 Beta Human Recombinant
Cat. No.
BT1967
Source
Escherichia Coli.
Synonyms
OSTB, OSTBETA, Organic solute transporter subunit beta, OST-beta, Solute carrier family 51 subunit beta.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SLC51B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 95 amino acids (57-128 a.a) and having a molecular mass of 10.7kDa.
SLC51B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The Solute Carrier Family 51 Beta (SLC51B) gene is responsible for encoding the SLC51B protein, which functions as a subunit of the organic solute transporter SLC51. SLC51 requires two distinct proteins to form a heterodimer for transport activity. However, the specific roles of each subunit in this process are not fully understood. Research indicates that SLC51B is crucial for the proper trafficking of SLC51A and the formation of a functional transport unit. Additionally, specific amino acid residues within SLC51B have been identified as essential for these functions. SLC51B has been linked to diseases such as extrahepatic cholestasis and biliary atresia.
Description
Recombinant SLC51B protein, produced in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein consists of 95 amino acids (spanning positions 57-128) and has a molecular weight of 10.7 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of the SLC51B protein, and proprietary chromatographic techniques are employed.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The SLC51B protein solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.1 M NaCl.
Stability
For optimal storage, it is recommended to keep the entire vial at 4°C if it will be used within 2-4 weeks. For extended storage, the protein should be stored frozen at -20°C. To ensure long-term stability during storage, consider adding a carrier protein (0.1% HSA or BSA). It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of the SLC51B protein is determined to be greater than 95.0% based on SDS-PAGE analysis.
Synonyms
OSTB, OSTBETA, Organic solute transporter subunit beta, OST-beta, Solute carrier family 51 subunit beta.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSRSIQASR KEKMQPPEKE TPEVLHLDEA KDHNSLNNLR ETLLSEKPNL AQVELELKER DVLSVFLPDV PETES

Product Science Overview

Gene and Protein Structure

The SLC51B gene is located on chromosome 15 in humans and encodes the beta subunit of the organic solute transporter (OST) complex. The OST complex is a heterodimer composed of two subunits: OST-alpha (SLC51A) and OST-beta (SLC51B). The beta subunit is essential for the proper functioning of the OST complex, as it modulates the glycosylation, membrane trafficking, and stability of the alpha subunit .

Function and Mechanism

The primary function of the OST complex is to facilitate the export of bile acids from enterocytes (intestinal cells) into the portal blood, which is a critical step in the enterohepatic circulation of bile acids. The OST complex efficiently transports major bile acid species, such as taurocholate, and can also transport steroids like estrone 3-sulfate and dehydroepiandrosterone 3-sulfate. Additionally, it plays a role in the transport of eicosanoids, such as prostaglandin E2 .

Biological Significance

The proper functioning of the SLC51B gene is vital for maintaining bile acid homeostasis and overall metabolic health. Mutations or dysregulation of this gene can lead to various disorders, including bile acid malabsorption and visceral myopathy. The OST complex’s ability to transport a wide range of substrates highlights its importance in multiple physiological processes, including lipid metabolism and steroid hormone regulation .

Recombinant SLC51B

Recombinant human SLC51B is produced using advanced biotechnological methods to study its structure, function, and potential therapeutic applications. The recombinant protein is typically expressed in suitable host cells, purified, and used in various research applications, including functional assays, structural studies, and drug development .

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