Shiga Like Toxin 1B Antibody
Greater than 95% as determined by 12% PAGE (coomassie staining).
Description
Product Specs
The purity of this antibody is greater than 95%, as determined by SDS-PAGE analysis with Coomassie blue staining.
Product Science Overview
Shiga-like toxins, also known as verotoxins, are a group of toxins produced by certain strains of Escherichia coli (E. coli), particularly E. coli O157:H7. These toxins are structurally and functionally similar to the Shiga toxin produced by Shigella dysenteriae. Shiga-like toxins are classified into two main types: Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2), with several subtypes within each category. The B subunit of Shiga-like toxin 1 (Stx1B) is responsible for binding to the host cell receptors, facilitating the entry of the toxin into the cells.
Shiga-like toxin 1B (Stx1B) is a pentameric protein composed of five identical subunits. Each subunit has a molecular weight of approximately 7.7 kDa. The B subunit binds to the glycolipid receptor globotriaosylceramide (Gb3) on the surface of host cells. This binding is crucial for the internalization of the toxin into the cells, where the A subunit exerts its toxic effects by inhibiting protein synthesis, leading to cell death.
Mouse anti Shiga-like toxin 1B antibodies are monoclonal antibodies produced by immunizing mice with purified recombinant Stx1B. The immunization process involves injecting the mice with the antigen (Stx1B) and adjuvants to enhance the immune response. After several booster injections, the spleen cells of the immunized mice are harvested and fused with myeloma cells to create hybridoma cells. These hybridoma cells are screened for the production of antibodies specific to Stx1B. The selected hybridoma clones are then cultured to produce large quantities of the monoclonal antibodies.
Mouse anti Shiga-like toxin 1B antibodies have several applications in scientific research and diagnostics:
- Enzyme-Linked Immunosorbent Assay (ELISA): These antibodies are used in ELISA to detect the presence of Shiga-like toxin 1B in various samples. The high specificity and sensitivity of monoclonal antibodies make them ideal for this application.
- Western Blotting: Mouse anti Shiga-like toxin 1B antibodies are used in Western blotting to identify and quantify the presence of Stx1B in protein samples. This technique is widely used in research to study the expression and regulation of Shiga-like toxins.
- Neutralization Assays: These antibodies can neutralize the toxic effects of Stx1B, making them valuable tools in studying the mechanisms of toxin action and in developing therapeutic interventions.
- Surface Plasmon Resonance (SPR): SPR is a technique used to study the interactions between molecules. Mouse anti Shiga-like toxin 1B antibodies can be used in SPR to investigate the binding kinetics and affinity of Stx1B to its receptors or other interacting molecules.
