SHH Rat
Greater than 95.0% as determined by SDS-PAGE.
Description
Sonic HedgeHog Recombinant Rat produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.9kDa.
SHH is purified by proprietary chromatographic techniques.
Product Specs
MIIGPGRGFG KRQHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKARI HCSVKAENSV AAKSDG.
Product Science Overview
Sonic HedgeHog (Shh) is a protein that plays a crucial role in the development of various tissues and organs in vertebrates. It is part of the Hedgehog signaling pathway, which is essential for embryonic development. The name “Sonic HedgeHog” was inspired by the popular video game character, reflecting the protein’s significant impact on developmental biology.
Sonic HedgeHog is a secreted protein that undergoes several post-translational modifications to become fully active. It is initially synthesized as a precursor protein, which is then cleaved to produce an active N-terminal fragment. This fragment is responsible for the protein’s signaling activity. The active form of Sonic HedgeHog binds to its receptor, Patched, on the surface of target cells, initiating a signaling cascade that regulates gene expression.
Sonic HedgeHog is expressed in various embryonic tissues, including the notochord, floor plate, and limb buds. It is critical for the patterning of the developing central nervous system, somites, and limbs. Additionally, Sonic HedgeHog is involved in the development of whiskers, hair, foregut, teeth, and bones .
Recombinant Sonic HedgeHog proteins are produced using genetic engineering techniques. These proteins are often expressed in bacterial or mammalian cell systems to ensure proper folding and post-translational modifications. Recombinant Sonic HedgeHog is used in various research applications, including studies on cell differentiation, tissue regeneration, and cancer biology.
- Cell Differentiation: Sonic HedgeHog signaling is crucial for the differentiation of various cell types, including neural progenitors and limb bud cells. Researchers use recombinant Sonic HedgeHog to study these processes in vitro.
- Tissue Regeneration: Sonic HedgeHog has been shown to promote the regeneration of damaged tissues, such as skin and bone. Recombinant Sonic HedgeHog is used in experiments to understand the underlying mechanisms and potential therapeutic applications.
- Cancer Biology: Aberrant Sonic HedgeHog signaling is implicated in the development of several cancers, including basal cell carcinoma and medulloblastoma. Researchers use recombinant Sonic HedgeHog to investigate the role of this pathway in tumorigenesis and to develop targeted therapies.
