SHH (C24II) Human
Description
The SHH is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Sonic Hedgehog (Shh) is a protein that plays a crucial role in the development of various tissues and organs in the human body. It is part of the Hedgehog signaling pathway, which is essential for embryonic development. The recombinant form of this protein, specifically the C24II variant, is used in various research and therapeutic applications.
The Sonic Hedgehog protein is composed of an N-terminal signaling domain and a C-terminal autoprocessing domain. The C24II variant refers to a specific form of the protein that includes the amino acids from cysteine at position 24 to glycine at position 197, with an N-terminal methionine. This variant is produced using recombinant DNA technology in E. coli .
The primary function of Shh is to regulate the growth and patterning of different tissues during embryonic development. It is involved in the formation of the central nervous system, somites, limbs, and various other structures . Shh signaling is also crucial for the development of whiskers, hair, foregut, teeth, and bones .
Sonic Hedgehog exerts its effects by binding to the Patched (Ptch) receptor on the cell surface. This binding relieves the inhibition on another protein called Smoothened (Smo), which then activates the downstream signaling cascade. This cascade ultimately leads to the activation of Gli transcription factors, which regulate the expression of target genes involved in cell proliferation, differentiation, and tissue patterning .
Recombinant Sonic Hedgehog (C24II) is widely used in research to study its role in development and disease. It is also used in various assays to induce specific cellular responses. For example, it can induce alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells, with an effective dose (ED50) of 0.1-0.4 µg/mL .
