SH3GLB1 Human
SH3-domain GRB2-like endophilin B1 Human Recombinant
Cat. No.
BT25916
Source
E.coli.
Synonyms
Bif-1, CGI-61, dJ612B15.2, PPP1R70, Endophilin-B1, Bax-interacting factor 1, SH3 domain-containing GRB2-like protein B1, KIAA0491 .
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SH3GLB1 Human Recombinant produced in E. coli is a single polypeptide chain containing 373 amino acids (1-365) and having a molecular mass of 41.9 kDa. SH3GLB1 is fused to 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Endophilin-B1 (SH3GLB1), a member of the endophilin family, is highly expressed in the heart, skeletal muscle, kidney, and placenta. This protein belongs to the B subgroup of the endophilin family, crucial for maintaining mitochondrial morphology and regulating the outer mitochondrial membrane dynamics. SH3GLB1 is essential for the proper functioning of these dynamics. Moreover, it plays a role in coatomer-mediated retrograde transport within certain cells. SH3GLB1 interacts with SH3GLB2 and Bcl-2-associated X protein, participating in the regulation of apoptotic signaling pathways.
Description
Recombinant human SH3GLB1, produced in E. coli, is a single polypeptide chain consisting of 373 amino acids (residues 1-365). With a molecular weight of 41.9 kDa, it includes an 8-amino acid His-tag fused at the C-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The SH3GLB1 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 10% glycerol, and 2mM DTT.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For longer storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. To maintain the protein's integrity, avoid repeated freezing and thawing.
Purity
The purity of SH3GLB1 is determined by SDS-PAGE analysis and is consistently greater than 90%.
Synonyms
Bif-1, CGI-61, dJ612B15.2, PPP1R70, Endophilin-B1, Bax-interacting factor 1, SH3 domain-containing GRB2-like protein B1, KIAA0491 .
Source
E.coli.
Amino Acid Sequence
MNIMDFNVKK LAADAGTFLS RAVQFTEEKL GQAEKTELDA HLENLLSKAE CTKIWTEKIM KQTEVLLQPN PNARIEEFVY EKLDRKAPSR INNPELLGQY MIDAGTEFGP GTAYGNALIK CGETQKRIGT ADRELIQTSA LNFLTPLRNF IEGDYKTIAK ERKLLQNKRL DLDAAKTRLK KAKAAETRNS SEQELRITQS EFDRQAEITR LLLEGISSTH AHHLRCLNDF VEAQMTYYAQ CYQYMLDLQK QLGSFPSNYL SNNNQTSVTP VPSVLPNAIG SSAMASTSGL VITSPSNLSD LKECSGSRKA RVLYDYDAAN STELSLLADE VITVFSVVGM DSDWLMGERG NQKGKVPITY LELLNLEHHH HHH.
Product Science Overview
Structure and Domains
SH3GLB1 contains several important domains:
- N-terminal domain: This region is essential for the interaction with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax).
- Central coiled-coil region: This region is necessary for the formation of homodimers and heterodimers with other proteins, such as SH3GLB2.
- C-terminal SH3 domain: This domain is involved in protein-protein interactions and is characteristic of the SRC homology 3 (SH3) domain-containing proteins .
Function
SH3GLB1 is implicated in several cellular functions:
- Mitochondrial Dynamics: It is required for normal outer mitochondrial membrane dynamics, including mitochondrial fission and fusion .
- Autophagy: SH3GLB1 is involved in the regulation of autophagy by activating lipid kinase activity of PIK3C3 during autophagy, probably by associating with the PI3K complex II (PI3KC3-C2) .
- Apoptosis: It promotes caspase-dependent apoptosis by activating BAX/BAK1 and is also involved in caspase-independent apoptosis during nutritional starvation .
Interaction with Other Proteins
SH3GLB1 interacts with several proteins to mediate its functions:
Expression and Localization
Clinical Significance
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