SH3BGRL3 Human

SH3 Domain Binding Glutamic Acid-Rich Protein Like 3 Human Recombinant
Cat. No.
BT25720
Source
E.coli.
Synonyms
SH3 Domain Binding Glutamic Acid-Rich Protein Like 3, SH3BGRL3-Like Protein, SH3 Domain-Binding Protein 1, SH3BP-1, TNF Inhibitory Protein, TIP-B1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SH3BGRL3 Human Recombinant produced in E. coli is a single polypeptide chain containing 116 amino acids (1-93) and having a molecular mass of 12.8 kDa.
SH3BGRL3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
SH3 domain-binding glutamic acid-rich-like protein 3 (SH3BGRL3), situated on chromosome 1p34.3-35, encodes a compact protein consisting of 93 amino acids. As the latest addition to the thioredoxin superfamily, SH3BGRL3's post-translationally modified form is recognized as TNF-alpha inhibitory protein. SH3BGRL3 potentially regulates the all-trans retinoic acid-induced pathway and modulates glutaredoxin biological activity.
Description
Recombinant human SH3BGRL3, produced in E. coli, is a single polypeptide chain comprising 116 amino acids (residues 1-93) with a molecular weight of 12.8 kDa. A 23 amino acid His-tag is fused to the N-terminus of SH3BGRL3. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
Sterile, colorless solution that has been filtered.
Formulation
The SH3BGRL3 solution (concentration: 1mg/ml) is prepared in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 1mM DTT, and 10% glycerol.
Stability
For optimal use within 2-4 weeks, store the solution at 4°C. For extended storage, freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is suggested for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 95% as assessed by SDS-PAGE.
Synonyms
SH3 Domain Binding Glutamic Acid-Rich Protein Like 3, SH3BGRL3-Like Protein, SH3 Domain-Binding Protein 1, SH3BP-1, TNF Inhibitory Protein, TIP-B1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSGLRVY STSVTGSREI KSQQSEVTRI LDGKRIQYQL VDISQDNALR DEMRALAGNP KATPPQIVNG DQYCGDYELF VEAVEQNTLQ EFLKLA

Product Science Overview

Introduction

SH3 Domain Binding Glutamic Acid-Rich Protein Like 3 (SH3BGRL3) is a protein encoded by the SH3BGRL3 gene in humans. This protein is part of the SH3BGR family, which includes small proteins that share similarities with the N-terminal region of the SH3BGR protein. SH3BGRL3 is a protein-coding gene and is known for its involvement in various biological processes and potential implications in diseases.

Gene and Protein Structure

The SH3BGRL3 gene is located on chromosome 1 at the position 1p36.11 . The protein encoded by this gene has a molecular weight of approximately 10.5 kDa and an isoelectric point of 5.0 . SH3BGRL3 shows significant similarity to glutaredoxin 1 of E. coli, and it is predicted to belong to the thioredoxin-like protein family .

Expression and Tissue Distribution

SH3BGRL3 is widely expressed in various human tissues, including monocytes, lungs, aorta, spleen, and skin . The protein is also found in the nucleus, extracellular exosomes, cytoplasm, and nuclear bodies . Its expression pattern suggests a role in diverse cellular functions.

Biological Functions

Although the precise function of SH3BGRL3 is not fully understood, it is believed to act as a modulator of glutaredoxin biological activity . It may also play a role in cytoskeleton organization . SH3BGRL3 lacks the enzymatic function of glutaredoxins but may serve as a regulator of redox activity .

Role in Disease

SH3BGRL3 has been associated with several diseases, including Three M Syndrome 1 and Myeloproliferative Neoplasm . It has also been found to be overexpressed in various tumors, such as glioblastoma multiforme and acute promyelocytic leukemia . The protein’s upregulation in these cancers suggests a potential role in tumorigenesis and cancer progression.

Interaction with Other Proteins

SH3BGRL3 has been shown to bind to myosin 1c in a calcium-dependent manner, indicating a possible role in modulating cytoskeletal dynamics . Additionally, recent studies suggest a relationship between SH3BGRL3 and members of the EGFR family, which are known to be involved in cell signaling and cancer .

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