SF20 Mouse

Chromosome 19 Open Reading Frame 10 Mouse Recombinant
Cat. No.
BT10288
Source
Escherichia Coli.
Synonyms
UPF0556 protein C19orf10 homolog, Stromal cell-derived growth factor SF20, Interleukin-25, IL-25, D17Wsu104e, Il25, IL27, SF20, IL27w, R33729_1, C19orf10, Chromosome 19 Open Reading Frame 10.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Chromosome 19 Open Reading Frame 10 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 143 amino acids and having a molecular mass of 15.8kDa. The Mouse SF20 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Mouse SF20, a growth factor derived from bone marrow stroma, is primarily found in the bone marrow and spleen stroma cells. Its presence is also observed in resting mononuclear cells, including resting CD8+ and CD19+ cells, as well as in activated CD8+ T cells. SF20 interacts with cells by binding to the surface receptor TSA-1 (Thymic shared Ag-1). This growth factor plays a role in stimulating the proliferation of FDCP2 cells (a mouse factor-dependent hemopoietic cell line) and mouse lymphoid cells.
Description
Recombinant Mouse Chromosome 19 Open Reading Frame 10, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 143 amino acids, resulting in a molecular mass of 15.8 kDa. The purification of Mouse SF20 is achieved using proprietary chromatographic techniques.
Physical Appearance
The product appears as a sterile, filtered, white, lyophilized (freeze-dried) powder.
Formulation
The lyophilization of Mouse SF20 was carried out from a 0.2µm filtered concentrated solution (1.0mg/ml) in PBS with a pH of 7.4.
Solubility
To reconstitute the lyophilized Mouse SF20, it is recommended to dissolve it in sterile water at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Mouse SF20 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it in a desiccated state below -18°C. After reconstitution, Mouse SF20 should be stored at 4°C for 2-7 days. For future use, it should be stored below -18°C. To ensure long-term stability, consider adding a carrier protein such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is determined using two methods: RP-HPLC analysis and SDS-PAGE analysis. The results indicate a purity greater than 97.0%.
Synonyms
UPF0556 protein C19orf10 homolog, Stromal cell-derived growth factor SF20, Interleukin-25, IL-25, D17Wsu104e, Il25, IL27, SF20, IL27w, R33729_1, C19orf10, Chromosome 19 Open Reading Frame 10.
Source
Escherichia Coli.
Amino Acid Sequence
MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL.

Product Science Overview

Expression and Function

C19orf10 is expressed in various tissues, including the bone marrow, spleen stroma cells, resting mononuclear cells, and activated CD8+ T cells . It plays a crucial role in the immune system, particularly in the regulation of T-cell responses. The protein is involved in the growth and differentiation of stromal cells, which are essential for the formation and maintenance of the bone marrow microenvironment .

Recombinant Protein

The recombinant form of C19orf10 is produced using genetic engineering techniques. This involves inserting the gene encoding C19orf10 into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. The recombinant protein is then purified and used for various research and therapeutic purposes .

Research and Applications

Research on C19orf10 has provided insights into its role in immune regulation and its potential therapeutic applications. Studies have shown that C19orf10 can modulate the activity of immune cells, making it a potential target for immunotherapy. Additionally, the protein’s involvement in stromal cell growth and differentiation suggests its potential use in regenerative medicine and tissue engineering .

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