SF20 Human

Chromosome 19 Open Reading Frame 10 Human Recombinant
Cat. No.
BT10186
Source
Escherichia Coli.
Synonyms
C19orf10, Interleukin-25, IL-25, IL25, IL27, IL-27, IL27w, IL-27w, Stromal cell-derived growth factor SF20, UPF0556 protein C19orf10, chromosome 19 open reading frame 10.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

SF20 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 162 amino acids fragment (33-173) and having a total molecular mass of 18 kDa. C9orf10 is fused to 20 amino acids His tag at N-terminus and is purified by proprietary chromatographic techniques.

Product Specs

Introduction
The interleukin SF20 is believed to be involved in the multiplication of lymphoid cells. Initially, it was discovered as a substance produced by stromal cells in the bone marrow.
Description
Produced in E. coli, Recombinant Human SF20 is a single, non-glycosylated polypeptide chain. It consists of 162 amino acids (fragment 33-173), resulting in a molecular mass of 18 kDa. The protein, also known as C9orf10, is fused to a 20 amino acid His tag at its N-terminus and undergoes purification using proprietary chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
C9orf10 is provided in a solution containing 20mM Tris HCL at a pH of 8 and 20% glycerol.
Stability
For optimal storage, keep the entire vial at 4°C if it will be used within 2-4 weeks. For extended storage, freeze at -20°C. Repeated freezing and thawing should be avoided.
Purity
SDS-PAGE analysis indicates a purity exceeding 95.0%.
Synonyms
C19orf10, Interleukin-25, IL-25, IL25, IL27, IL-27, IL27w, IL-27w, Stromal cell-derived growth factor SF20, UPF0556 protein C19orf10, chromosome 19 open reading frame 10.
Source
Escherichia Coli.

Product Science Overview

Gene and Protein Structure

The C19orf10 gene is situated on the short arm of chromosome 19. It encodes a protein that is composed of a specific sequence of amino acids, which are the building blocks of proteins. The exact function of the C19orf10 protein is not fully understood, but it is believed to play a role in various cellular processes.

Expression and Function

The expression of the C19orf10 gene varies across different tissues in the human body. It is known to be expressed in several tissues, including the liver, kidney, and brain. The protein encoded by this gene is thought to be involved in cellular signaling pathways, although the precise mechanisms remain to be elucidated.

Recombinant Protein Production

Recombinant human C19orf10 is produced using recombinant DNA technology. This involves inserting the C19orf10 gene into a suitable expression vector, which is then introduced into a host cell, such as bacteria or yeast. The host cells are cultured under conditions that promote the expression of the C19orf10 protein. Once produced, the protein is purified from the host cells for use in various research applications.

Research Applications

Recombinant human C19orf10 is used in a variety of research studies to understand its function and role in human health and disease. It is particularly useful in studies involving protein-protein interactions, cellular signaling pathways, and the identification of potential therapeutic targets. Researchers use recombinant proteins to study the effects of specific mutations, post-translational modifications, and interactions with other cellular components.

Clinical Relevance

While the exact clinical significance of C19orf10 is still under investigation, alterations in the expression or function of this protein may be associated with certain diseases. Understanding the role of C19orf10 in disease processes could lead to the development of new diagnostic and therapeutic strategies.

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