SERPING1 Human, Sf9
Sf9, Baculovirus cells.
Serpin Family G Member 1, Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1, Plasma Protease C1 Inhibitor, C1 Esterase Inhibitor, C1-Inhibiting Factor, Serpin G1, C1NH, C1IN, Serine (Or Cysteine) Proteinase Inhibitor, Clade G (C1 Inhibitor), Member 1, (Angioedema, Hereditary), Serine/Cysteine Proteinase Inhibitor Clade G Member 1, Serpin Peptidase Inhibitor Clade G Member 1, Complement Component 1 Inhibitor, Angioedema, Hereditary, C1 Inh, C1INH, C1Inh, HAE1, HAE2.
Greater than 90.0% as determined by SDS-PAGE.
Description
SERPING1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 489 amino acids (23-500a.a.) and having a molecular mass of 54.2kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
SERPING1 is expressed with an 11 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Plasma protease C1 inhibitor (SERPING1) belongs to the serpin superfamily of serine protease inhibitors, playing a critical role in regulating the complement and contact systems. This regulatory function involves controlling the activation of complement factor C1 and inhibiting the activity of activated C1 by binding to the active catalytic site of C1r and C1s light chains. SERPING1 deficiency leads to hereditary angioedema, a condition marked by recurring episodes of localized swelling (angioedema) affecting the skin, gastrointestinal mucosa, or upper respiratory mucosa.
Produced in Sf9 Baculovirus cells, SERPING1 is a single, glycosylated polypeptide chain comprising 489 amino acids (23-500a.a.) with a molecular mass of 54.2kDa. On SDS-PAGE, its molecular size appears approximately between 70-100kDa. The protein is expressed with an 11 amino acid His tag at the C-Terminus and purified using proprietary chromatographic techniques.
The SERPING1 protein solution is provided at a concentration of 1mg/ml and contains 10% glycerol and Phosphate Buffered Saline (pH 7.4).
The purity of SERPING1 is determined to be greater than 90.0% by SDS-PAGE analysis.
Serpin Family G Member 1, Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1, Plasma Protease C1 Inhibitor, C1 Esterase Inhibitor, C1-Inhibiting Factor, Serpin G1, C1NH, C1IN, Serine (Or Cysteine) Proteinase Inhibitor, Clade G (C1 Inhibitor), Member 1, (Angioedema, Hereditary), Serine/Cysteine Proteinase Inhibitor Clade G Member 1, Serpin Peptidase Inhibitor Clade G Member 1, Complement Component 1 Inhibitor, Angioedema, Hereditary, C1 Inh, C1INH, C1Inh, HAE1, HAE2.
Sf9, Baculovirus cells.
ADPEFNPNAT SSSSQDPESL QDRGEGKVAT TVISKMLFVE PILEVSSLPT TNSTTNSATK ITANTTDEPT TQPTTEPTTQ PTIQPTQPTT QLPTDSPTQP TTGSFCPGPV TLCSDLESHS TEAVLGDALV DFSLKLYHAF SAMKKVETNM AFSPFSIASL LTQVLLGAGE NTKTNLESIL SYPKDFTCVH QALKGFTTKG VTSVSQIFHS PDLAIRDTFV NASRTLYSSS PRVLSNNSDA NLELINTWVA KNTNNKISRL LDSLPSDTRL VLLNAIYLSA KWKTTFDPKK TRMEPFHFKN SVIKVPMMNS KKYPVAHFID QTLKAKVGQL QLSHNLSLVI LVPQNLKHRL EDMEQALSPS VFKAIMEKLE MSKFQPTLLT LPRIKVTTSQ DMLSIMEKLE FFDFSYDLNL CGLTEDPDLQ
VSAMQHQTVL ELTETGVEAA AASAISVART LLVFEVQQPF LFVLWDQQHK FPVFMGRVYD PRAHHHHHH.
Product Science Overview
Serpin Peptidase Inhibitor, Clade G Member 1, also known as SERPING1, is a protein encoded by the SERPING1 gene in humans. This protein is a member of the serpin (serine protease inhibitor) superfamily and plays a crucial role in regulating various physiological pathways, including the complement system, blood coagulation, fibrinolysis, and the generation of kinins .
The SERPING1 gene is located on chromosome 11q12.1 and encodes a highly glycosylated plasma protein known as C1 inhibitor (C1-INH) . The protein consists of 478 amino acids and has a molecular weight of approximately 52 kDa. It is synthesized primarily in the liver and secreted into the bloodstream .
C1 inhibitor (C1-INH) is a key regulator of the complement system, which is part of the immune response. It inhibits the activated forms of C1r and C1s, which are components of the first complement component (C1). By doing so, C1-INH prevents the uncontrolled activation of the complement cascade, which could otherwise lead to tissue damage .
In addition to its role in the complement system, C1-INH also inhibits other proteases, including kallikrein, factor XIIa, and chymotrypsin. This broad inhibitory activity allows C1-INH to regulate various physiological processes, such as blood coagulation and fibrinolysis .
Deficiency or dysfunction of C1-INH is associated with a rare genetic disorder known as hereditary angioedema (HAE). HAE is characterized by recurrent episodes of severe swelling (angioedema) in various parts of the body, including the skin, gastrointestinal tract, and airways. This condition can be life-threatening if it leads to airway obstruction .
There are two main types of HAE:
To address the therapeutic needs of patients with HAE, recombinant forms of C1-INH have been developed. One such form is Human Recombinant C1-INH produced in Sf9 insect cells. This recombinant protein is designed to mimic the natural C1-INH and is used to prevent and treat acute attacks of angioedema in patients with HAE .
