SERPINC1 Human, Sf9
Sf9, Baculovirus cells.
SERPINC1, AT3, AT3D, ATIII, THPH7, Antithrombin-III, Serpin C1.
Greater than 90.0% as determined by SDS-PAGE.
Description
SERPINC Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 441 amino acids (33-464a.a.) and having a molecular mass of 50.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). SERPINC is expressed with a 9 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
SERPINC1, AT3, AT3D, ATIII, THPH7, Antithrombin-III, Serpin C1.
Sf9, Baculovirus cells.
ADPHGSPVDI CTAKPRDIPM NPMCIYRSPE KKATEDEGSE QKIPEATNRR VWELSKANSR FATTFYQHLA DSKNDNDNIF LSPLSISTAF AMTKLGACND TLQQLMEVFK FDTISEKTSD QIHFFFAKLN CRLYRKANKS SKLVSANRLF GDKSLTFNET YQDISELVYG AKLQPLDFKE NAEQSRAAIN KWVSNKTEGR ITDVIPSEAI NELTVLVLVN TIYFKGLWKS KFSPENTRKE LFYKADGESC SASMMYQEGK FRYRRVAEGT QVLELPFKGD DITMVLILPK PEKSLAKVEK ELTPEVLQEW LDELEEMMLV VHMPRFRIED GFSLKEQLQD MGLVDLFSPE KSKLPGIVAE GRDDLYVSDA FHKAFLEVNE EGSEAAASTA VVIAGRSLNP NRVTFKANRP FLVFIREVPL NTIIFMGRVA NPCVKHHHHH H.
Product Science Overview
Serpin Peptidase Inhibitor, Clade C Member 1, also known as SERPINC1 or antithrombin III, is a crucial protein in the human body. It belongs to the serpin (serine protease inhibitor) superfamily, which plays a significant role in regulating various physiological processes, including blood coagulation, inflammation, and immune responses .
SERPINC1 is a plasma protease inhibitor that primarily inhibits thrombin and other activated serine proteases involved in the coagulation cascade. This inhibition is essential for maintaining the balance between coagulation and anticoagulation, preventing excessive clot formation . The protein is composed of 455 amino acids and has a molecular mass of approximately 51.4 kDa .
The recombinant form of SERPINC1 is produced using the Sf9 insect cell expression system. This system is widely used for producing recombinant proteins due to its ability to perform post-translational modifications similar to those in mammalian cells. The recombinant SERPINC1 is typically purified using chromatographic techniques to ensure high purity and activity .
Deficiencies in SERPINC1 can lead to antithrombin III deficiency, an autosomal dominant disorder that increases the risk of thrombosis. This condition can result in recurrent deep vein thrombosis, pulmonary embolism, and other thrombotic events . Recombinant SERPINC1 is used therapeutically to manage and treat patients with antithrombin III deficiency, providing a crucial tool in preventing and controlling thrombotic disorders .
Recombinant SERPINC1 has several applications in research and medicine:
- Therapeutic Use: It is used to treat patients with antithrombin III deficiency, helping to prevent thrombotic events.
- Research: It serves as a valuable tool in studying the mechanisms of blood coagulation and the role of serpins in various physiological processes.
- Biotechnology: The recombinant protein is used in the development of anticoagulant therapies and other biotechnological applications .
