SEPT5 Human
Description
The SEPT5 is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Septin-5 is a member of the septin family of GTP-binding proteins, which are highly conserved across species, including yeast, Drosophila, and mammals . Septins play crucial roles in various cellular processes, such as cytokinesis, cell polarity, and vesicle trafficking. Septin-5, in particular, has been implicated in the regulation of cytoskeletal organization and neurotransmitter release.
The SEPTIN5 gene encodes the Septin-5 protein, which is involved in several cellular pathways, including the regulation of activated PAK-2p34 by proteasome-mediated degradation and ERK signaling . The gene is located on chromosome 22q11.2, a region associated with DiGeorge syndrome and other developmental disorders .
Septin-5 is known to interact with other septin proteins to form hetero-oligomeric complexes that can assemble into filaments and higher-order structures. These structures are essential for maintaining cell shape, polarity, and intracellular transport. In neurons, Septin-5 is involved in the regulation of synaptic vesicle exocytosis, which is critical for neurotransmitter release .
Mutations or dysregulation of the SEPTIN5 gene have been associated with various neurological disorders. For instance, autoantibodies targeting Septin-5 have been identified in patients with autoimmune cerebellar ataxia, a condition characterized by loss of balance, incoordination, and eye movement abnormalities . This autoimmune response can lead to significant neurological impairment, but some patients have shown improvement with immunotherapy .
Recombinant human Septin-5 is produced using molecular cloning techniques, where the SEPTIN5 gene is inserted into an expression vector and introduced into a host cell system, such as E. coli or mammalian cells. The recombinant protein is then purified for use in various research applications, including studies on protein-protein interactions, cellular signaling pathways, and the development of therapeutic interventions .
Research on Septin-5 has provided valuable insights into its role in cellular processes and its potential as a therapeutic target. Studies have shown that Septin-5 is involved in the regulation of neurotransmitter release, making it a potential target for treating neurological disorders . Additionally, the use of recombinant Septin-5 in research has facilitated the study of its structure and function, contributing to our understanding of septin biology and its implications in health and disease .
