SEPT2 Human

Septin-2 Human Recombinant
Cat. No.
BT21872
Source
Escherichia Coli.
Synonyms
Septin 2, DIFF6, NEDD5, hNedd5, Pnutl3, NEDD-5.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

SEPT2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 381 amino acids (1-361 a.a.) and having a molecular mass of 43.6kDa. SEPT2 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
SEPT2, a GTPase essential for cytokinesis, plays a role in exocytosis. This protein can form hetero-oligomers with Septin6 and Septin7, contributing to the organization of new growth in organisms. SEPT2 is found in the core of Tau-based paired helical filaments and is involved in the formation of neurofibrillary tangles, acting as an integral part of these filaments.
Description
Recombinant human SEPT2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 381 amino acids (amino acids 1-361). It has a molecular weight of 43.6 kDa. The protein includes a 20 amino acid His Tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, sterile solution without any color.
Formulation
The SEPT2 solution is provided at a concentration of 0.25 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep the solution refrigerated at 4°C. For extended storage, freeze the solution at -20°C. To ensure long-term stability, consider adding a carrier protein (0.1% HSA or BSA) before freezing. Avoid repeated freeze-thaw cycles.
Purity
The purity level of the protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Septin 2, DIFF6, NEDD5, hNedd5, Pnutl3, NEDD-5.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSKQQPTQFI NPETPGYVGF ANLPNQVHRK SVKKGFEFTL MVVGESGLGK STLINSLFLT DLYPERVISG AAEKIERTVQ IEASTVEIEE RGVKLRLTVV DTPGYGDAIN CRDCFKTIIS YIDEQFERYL HDESGLNRRH IIDNRVHCCF YFISPFGHGL KPLDVAFMKA IHNKVNIVPV IAKADTLTLK ERERLKKRIL DEIEEHNIKI YHLPDAESDE DEDFKEQTRL LKASIPFSVV GSNQLIEAKG KKVRGRLYPW GVVEVENPEH NDFLKLRTML ITHMQDLQEV TQDLHYENFR SERLKRGGRK VENEDMNKDQ ILLEKEAELR RMQEMIARMQ AQMQMQMQGG DGDGGALGHH V.

Product Science Overview

Structure and Expression

Septin-2 is a filament-forming cytoskeletal GTPase. It forms a filamentous structure with other septins such as SEPT6, SEPT7, and SEPT12. This structure is crucial for the integrity and motility of the sperm tail during postmeiotic differentiation . The recombinant form of Septin-2 is typically expressed in Escherichia coli and is purified to a high degree of purity (>90%) using conventional chromatography techniques .

Functions

  1. Cytokinesis: Septin-2 plays a critical role in cytokinesis, the process by which a cell divides its cytoplasm to produce two daughter cells. It forms a scaffold at the midplane of the mitotic spindle, which is essential for maintaining the localization of CENPE at kinetochores and ensuring proper chromosome congression .

  2. Actin Cytoskeleton Organization: Septin-2 is required for the normal organization of the actin cytoskeleton. It helps maintain polyglutamylated microtubules, facilitating efficient vesicle transport and impeding MAP4 binding to tubulin .

  3. Ciliogenesis: In cilia, Septin-2 is necessary for the integrity of the diffusion barrier at the base of the primary cilium. This barrier prevents the diffusion of transmembrane proteins between the cilia and plasma membranes .

  4. Cell Movement: Septin-2 also plays a role in collective cell movements, which are essential for various developmental processes and wound healing .

Applications

Recombinant Septin-2 is used in various research applications, including:

  • SDS-PAGE: For analyzing protein purity and molecular weight.
  • Mass Spectrometry (MS): For identifying and quantifying proteins.
  • Functional Studies: To understand the role of Septin-2 in cellular processes .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.