SEP15 Human

15 KDa Selenoprotein Human Recombinant
Cat. No.
BT21661
Source
Escherichia Coli.
Synonyms
15 KDa Selenoprotein, SEP15.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

SEP15 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (29-165 a.a) and having a molecular mass of 17.7kDa.
SEP15 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The 15 KDa Selenoprotein (SEP15) is a protein-coding gene that contains a selenocysteine (Sec) residue at its active site. Selenocysteine is encoded by the UGA codon, which typically signals the termination of translation. The 3' UTR of selenoprotein genes has a standard stem-loop structure called the selenocysteine insertion sequence (SECIS). SECIS is required for the recognition of UGA as a Sec codon, rather than as a stop signal. Studies conducted on mice suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. The SEP15 gene is situated on chromosome 1p31, a genetic locus frequently mutated or deleted in human cancers. SEP15 has been linked to diseases like lung cancer susceptibility and chronic lymphocytic leukemia.
Description
Recombinant human SEP15, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 160 amino acids (29-165 a.a), has a molecular mass of 17.7 kDa, and is fused to a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
SEP15 protein solution at a concentration of 0.5mg/ml in a buffer composed of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is important to avoid repeated freezing and thawing.
Purity
SDS-PAGE analysis indicates a purity greater than 95.0%.
Synonyms
15 KDa Selenoprotein, SEP15.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSVSAFGAE FSSEACRELG FSSNLLCSSC DLLGQFNLLQ LDPDCRGCCQ EEAQFETKKL YAGAILEVCG CKLGRFPQVQ AFVRSDKPKL FRGLQIKYVR GSDPVLKLLD DNGNIAEELS ILKWNTDSVE EFLSEKLERI

Product Science Overview

Introduction

The 15 kDa selenoprotein, also known as SEP15, is one of the more than 25 selenoproteins identified in humans. Selenoproteins are unique proteins that incorporate the amino acid selenocysteine, which is known for its role in redox regulation and antioxidant defense mechanisms. SEP15 has garnered significant interest due to its potential implications in human health and disease, particularly in cancer biology.

Gene and Structure

The SEP15 gene is located on human chromosome 1 at position p31 . This gene spans approximately 51 kilobases and consists of five exons . The protein encoded by this gene is composed of 162 amino acids and contains a single selenocysteine residue at position 93 . The selenocysteine insertion sequence (SECIS) element, which is essential for the incorporation of selenocysteine into the protein, is found in the 3′-untranslated region (UTR) of its mRNA .

Function and Localization

SEP15 is an endoplasmic reticulum (ER)-resident protein and is involved in the quality control of protein folding . It associates with UDP-glucose:glycoprotein glucosyltransferase (UGTR), a protein that plays a crucial role in the proper folding of glycoproteins . SEP15 is believed to function as an oxidoreductase, contributing to redox regulation within the ER .

Expression and Regulation

The expression of SEP15, like many other selenoproteins, is regulated by the selenium status of the organism . Under conditions of low selenium, the expression of inducible selenoproteins such as SEP15 is reduced, while essential housekeeping selenoproteins maintain their expression . SEP15 is expressed in a wide range of tissues, with the highest levels observed in the liver, kidney, testes, thyroid, and prostate .

Role in Cancer

SEP15 has been implicated in cancer biology due to its differential expression in normal and malignant tissues . Polymorphisms in the SEP15 gene have been linked to an increased risk of several types of cancer . The gene’s location on chromosome 1p31, a region often deleted or mutated in various cancers, further supports its potential role in cancer etiology . SEP15’s involvement in the chemopreventive effects of dietary selenium is also a subject of ongoing research .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

SEPX1 Human
Selenoprotein X 1 Human Recombinant
Cat. No.
BT21816
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.