SDC4 Human

Syndecan-4 Human Recombinant
Cat. No.
BT7242
Source
Escherichia Coli.
Synonyms
SDC-4, SYND4, SYND-4, Amphiglycan, Ryudocan core protein, Syndecan-4.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SDC4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids (19-145a.a.) and having a molecular mass of 13.9 kDa. SDC4 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Syndecan-4, a member of the syndecan family, is a type I integral membrane heparan sulfate proteoglycan (HSPG) initially identified as an antithrombin-binding molecule in cloned rat microvascular endothelial cells. It interacts with various molecules like basic fibroblast growth factor (bFGF), midkine, and tissue factor pathway inhibitor through its heparan sulfate chains, suggesting its involvement in diverse biological processes, including bFGF signaling, anticoagulation, and focal adhesion formation. Studies indicate widespread syndecan-4 expression, with notably high levels in the kidney, hinting at its role in renal function. Additionally, research implicates proteoglycans, particularly sulfated ones, in kidney organogenesis.
Description
Recombinant human SDC4, produced in E. coli, is an unglycosylated polypeptide chain comprising 128 amino acids (residues 19-145a.a.) with a molecular weight of 13.9 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
Syndecan-4 protein solution (1 mg/ml) in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 85% as assessed by SDS-PAGE.
Synonyms
SDC-4, SYND4, SYND-4, Amphiglycan, Ryudocan core protein, Syndecan-4.
Source
Escherichia Coli.
Amino Acid Sequence
MESIRETEVI DPQDLLEGRY FSGALPDDED VVGPGQESDD FELSGSGDLD DLEDSMIGPE VVHPLVPLDN HIPERAGSGS QVPTEPKKLE ENEVIPKRIS PVEESEDVSN KVSMSSTVQG SNIFERTE.

Product Science Overview

Introduction

Syndecan-4 is a member of the syndecan family of type I transmembrane heparan sulfate proteoglycans (HSPGs). These proteins are integral components of the cell surface and play crucial roles in cell-cell and cell-matrix interactions. Syndecan-4, in particular, is encoded by the SDC4 gene in humans and has a molecular weight of approximately 20 kDa .

Structure and Function

Syndecan-4 consists of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. The extracellular domain is capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans, which are essential for its function in binding to various ligands, including growth factors, cytokines, and extracellular matrix components . The cytoplasmic domain contains conserved regions that are crucial for signal transduction and interaction with the cytoskeleton .

Biological Roles

Syndecan-4 is involved in a variety of biological processes, including:

  • Cell Adhesion and Migration: It plays a significant role in cell adhesion and migration by interacting with integrins and other cell surface receptors .
  • Wound Healing: Syndecan-4 is upregulated during wound healing and is essential for the formation of focal adhesions and actin stress fibers .
  • Angiogenesis: It is involved in the regulation of angiogenesis by modulating the activity of growth factors such as fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) .
  • Inflammation: Syndecan-4 participates in the inflammatory response by regulating the activity of chemokines and cytokines .
Recombinant Human Syndecan-4

Recombinant human Syndecan-4 is produced using a mouse myeloma cell line, NS0, and is typically tagged with a C-terminal 6-His tag for purification purposes . The recombinant protein is used in various research applications, including binding assays, enzyme assays, and in vivo studies .

Applications in Research

Recombinant Syndecan-4 is utilized in several research areas:

  • Functional Studies: It is used to study the binding interactions with growth factors and other ligands in functional assays .
  • Cell Culture: The protein is used in cell culture experiments to investigate its role in cell adhesion, migration, and signaling .
  • Therapeutic Research: Syndecan-4 is being explored as a potential therapeutic target for diseases related to impaired wound healing, inflammation, and cancer .

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