SDC2 Human
Sf9, Baculovirus cells.
Syndecan 2, Heparan Sulfate Proteoglycan 1 Cell Surface-Associated, Heparan Sulfate Proteoglycan Core Protein, Syndecan Proteoglycan 2, Fibroglycan, HSPG1, SYND2, HSPG, Cell Surface-Associated Heparan Sulfate Proteoglycan 1, CD362 Antigen, Syndecan-2, CD362, SDC2.
Greater than 90.0% as determined by SDS-PAGE.
Description
SDC2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 135 amino acids (19-144a.a.) and having a molecular mass of 15kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). SDC2 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Syndecan 2 (SDC2) is a member of the syndecan family, which are transmembrane proteins that carry heparan sulfate and chondroitin sulfate. It plays a role in modulating the activity and movement of certain growth factor receptors and integrins. SDC2 is found in hepatic stellate cells (HSCs), which are important for matrix deposition in liver fibrosis. It is involved in the creation of specialized membrane domains and acts as a direct connection between the cell's external environment and the organization of the cytoplasm within the cell cortex.
This product consists of the recombinant human SDC2 protein produced in Sf9 Baculovirus cells. It is a single, glycosylated polypeptide chain with a molecular weight of 15 kDa (appears at approximately 18-28 kDa on SDS-PAGE). The protein sequence encompasses amino acids 19-144a.a. and includes a 6-amino acid His tag at the C-terminus. Purification is achieved using proprietary chromatographic techniques.
The SDC2 protein solution has a concentration of 0.25 mg/ml and is prepared in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Purity is determined to be greater than 90.0% based on SDS-PAGE analysis.
Syndecan 2, Heparan Sulfate Proteoglycan 1 Cell Surface-Associated, Heparan Sulfate Proteoglycan Core Protein, Syndecan Proteoglycan 2, Fibroglycan, HSPG1, SYND2, HSPG, Cell Surface-Associated Heparan Sulfate Proteoglycan 1, CD362 Antigen, Syndecan-2, CD362, SDC2.
Sf9, Baculovirus cells.
ADPESRAELT SDKDMYLDNS SIEEASGVYP IDDDDYASAS GSGADEDVES PELTTSRPLP KILLTSAAPK VETTTLNIQN KIPAQTKSPE ETDKEKVHLS DSERKMDPAE EDTNVYTEKH SDSLFKRTEH HHHHH.
Product Science Overview
Syndecan-2, also known as CD362, is a member of the syndecan family of heparan sulfate proteoglycans (HSPGs). These are transmembrane proteins that play crucial roles in cell proliferation, survival, adhesion, and migration. Syndecan-2 is particularly significant due to its involvement in various cellular processes and its potential as a therapeutic target.
Syndecan-2 consists of a core protein decorated with heparan sulfate chains. These chains interact with a variety of proteins, including growth factors, cytokines, and extracellular matrix components, facilitating numerous cellular functions . The protein is capable of carrying both heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans .
Syndecan-2 is involved in the regulation of stem cells, particularly hematopoietic stem cells (HSCs). It has been shown to enrich for HSCs and regulate their behavior in the bone marrow niche . The inhibition of heparan sulfate synthesis or the use of heparan sulfate mimetics can mobilize HSCs, suggesting a role for Syndecan-2 in the retention of these cells in the bone marrow .
Recombinant human Syndecan-2 is produced using various expression systems, including HEK293 cells and mouse myeloma cell lines . The recombinant protein is typically tagged with a polyhistidine tag at the C-terminus to facilitate purification. It is available in both carrier-free and carrier-containing formulations, depending on the intended application .
Recombinant Syndecan-2 is used in various research applications, including:
- Stem Cell Research: Understanding the role of Syndecan-2 in stem cell regulation and mobilization.
- Cancer Research: Investigating the potential of Syndecan-2 as a diagnostic biomarker and therapeutic target, particularly in colorectal cancer .
- Cell Signaling Studies: Exploring the interactions between Syndecan-2 and other cell surface receptors, such as growth factor receptors and integrins .
