SDC1 Human

Syndecan-1 Human Recombinant
Cat. No.
BT7078
Source
E.coli.
Synonyms
Heparan Sulfate proteoglycan fibroblast growth factor receptor, syndecan proteoglycan 1, Syndecan 1, SYND1, CD138 antigen, SDC.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SDC1 Human Recombinant produced in E. coli is a single polypeptide chain containing 262 amino acids (18-254) and having a molecular mass of 27kDa (molecular size on SDS-PAGE will appear higher).
SDC1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD138, also known as SDC1, plays a role in cell adhesion. It is found on the surface of pre-B cells and plasma cells but not on mature B cells. SDC1 serves as a specific marker for B cell lymphoblastic leukemia and lymphoplasmacytoid leukemia. Its absence from apoptotic myeloma cells makes it a useful marker for plasma cell and myeloma cells.
Description
Recombinant human SDC1, produced in E. coli, is a single polypeptide chain consisting of 262 amino acids (residues 18-254) with a molecular weight of 27kDa. Note that the molecular size on SDS-PAGE might appear higher. The SDC1 protein is fused to a 25 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The SDC1 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 100mM NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the SDC1 solution can be stored at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is advisable for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein stability.
Purity
The purity of the SDC1 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Heparan Sulfate proteoglycan fibroblast growth factor receptor, syndecan proteoglycan 1, Syndecan 1, SYND1, CD138 antigen, SDC.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMQPALP QIVATNLPPE DQDGSGDDSD NFSGSGAGAL QDITLSQQTP STWKDTQLLT AIPTSPEPTG LEATAASTST LPAGEGPKEG EAVVLPEVEP GLTAREQEAT PRPRETTQLP TTHQASTTTA TTAQEPATSH PHRDMQPGHH ETSTPAGPSQ ADLHTPHTED GGPSATERAA EDGASSQLPA AEGSGEQDFT FETSGENTAV VAVEPDRRNQ SPVDQGATGA SQGLLDRKEV LG.

Product Science Overview

Structure and Function

Syndecan-1 consists of a core protein with attached heparan sulfate and chondroitin sulfate chains. These glycosaminoglycan chains enable syndecan-1 to bind to a variety of ligands, including growth factors, chemokines, and extracellular matrix components . This binding capacity allows syndecan-1 to mediate several biological processes, such as:

  • Cell adhesion: Syndecan-1 facilitates the adhesion of cells to the extracellular matrix and to other cells .
  • Cell signaling: It acts as a co-receptor for various signaling pathways, including Wnt, hedgehog, and fibroblast growth factor (FGF) pathways .
  • Cytoskeletal organization: Syndecan-1 is involved in the organization of the cytoskeleton, which is essential for maintaining cell shape and motility .
Role in Disease

Syndecan-1 is implicated in several pathological conditions, including cancer, inflammation, and wound healing . In cancer, syndecan-1 is often misexpressed, and its levels can correlate with tumor progression and poor prognosis . It is also involved in the regulation of cancer stem cells, which are responsible for tumor recurrence and resistance to therapy .

Recombinant Syndecan-1

Recombinant human syndecan-1 is produced using various expression systems, such as mammalian cells, to ensure proper post-translational modifications and biological activity . The recombinant protein is used in research to study its role in cell adhesion, signaling, and disease mechanisms. It is also utilized in drug development and therapeutic applications .

Applications in Research and Medicine
  • Cancer Research: Syndecan-1 is studied for its role in tumor biology and as a potential therapeutic target .
  • Stem Cell Research: Its involvement in stem cell signaling pathways makes it a valuable tool for studying stem cell biology .
  • Wound Healing: Syndecan-1’s role in cell adhesion and migration is explored in the context of tissue repair and regeneration .

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