SAA2 Human
Escherichia Coli.
Serum amyloid A-2 protein, SAA2, Amyloid A2 protein.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95% as determined by SDS-PAGE.
Description
SAA2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing N-terminal Met and having a molecular mass of 11.76kDa. SAA2Human is purified by proprietary chromatographic techniques.
Product Specs
Recombinant human SAA2, produced in E. coli, is a single, non-glycosylated polypeptide chain containing an N-terminal methionine. It has a molecular mass of 11.76 kDa. SAA2 Human is purified using proprietary chromatographic techniques.
Sterile filtered white lyophilized (freeze-dried) powder.
The protein solution was filtered and concentrated, then lyophilized from a 0.01 M HCl solution (pH 2.0).
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to avoid repeated freezing and thawing cycles. The reconstituted protein can be stored at 4°C for a limited time.
Reconstitute the lyophilized SAA2 in 0.01 M HCl (pH 2.0) at a concentration of at least 100 µg/ml. It can then be further diluted in other aqueous solutions.
Greater than 95% purity as determined by SDS-PAGE.
Serum amyloid A-2 protein, SAA2, Amyloid A2 protein.
Escherichia Coli.
MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGAWAAEVIS NARENIQRLT GHGAEDSLAD QAANKWGRSG RDPNHFRPAG LPEKY.
Product Science Overview
Serum Amyloid A2 (SAA2) is a member of the serum amyloid A family of apolipoproteins. These proteins are primarily produced by hepatocytes in response to pro-inflammatory cytokines. SAA2, along with other members of the SAA family, plays a crucial role in the acute phase response to inflammation and tissue injury .
SAA2 is a small, non-glycosylated protein with a molecular weight of approximately 12 kDa . It is encoded by the SAA2 gene, which is one of several closely related genes in the SAA family . The protein is secreted as part of the high-density lipoprotein (HDL) complex and circulates in the bloodstream .
SAA2 has several important biological functions:
- Acute Phase Response: SAA2 is highly expressed during the acute phase response, a rapid inflammatory response to infection, injury, or other stressors. Its levels can increase up to 1000-fold within 24 hours of the onset of inflammation .
- Cytokine-like Activity: SAA2 functions as a cytokine-like protein, playing a role in cell-cell communication and feedback in inflammatory, immunologic, neoplastic, and protective pathways .
- Lipid Metabolism: Due to its lipophilicity, SAA2 is involved in lipid transport and metabolism. It is associated with HDL and can influence atherosclerosis development .
Recombinant SAA2 is produced using recombinant DNA technology, typically in bacterial expression systems such as E. coli . This allows for the production of large quantities of the protein for research and therapeutic purposes. Recombinant SAA2 retains the biological activity of the native protein and is used in various studies to understand its role in inflammation and other physiological processes .
SAA2 is a biomarker for inflammation and is used in clinical settings to monitor inflammatory diseases. Elevated levels of SAA2 are associated with various conditions, including infections, autoimmune diseases, and cancers . Understanding the regulation and function of SAA2 can provide insights into the pathophysiology of these diseases and potential therapeutic targets.
