S100A7 Human

S100 Calcium Binding Protein A7 Human Recombinant
Cat. No.
BT362
Source
Escherichia Coli.
Synonyms
S100 calcium-binding protein A7 (psoriasin 1), S100A7c, PSOR1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

S100A7 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 121 amino acids (1-101) and having a molecular mass of 13.6 kDa.
The S100A4 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
S100A7, a member of the S-100 calcium-binding protein family, is secreted into the cytoplasm via a non-classical pathway. This protein plays a role in regulating various cellular processes, including cell cycle progression, differentiation, and growth. Characterized by its two EF-hand domains, S100A7 exhibits significant upregulation in psoriatic epidermis, bladder squamous cell carcinoma, and breast cancer tissues, suggesting its potential involvement in carcinogenesis.
Description
Recombinant human S100A7, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 121 amino acids (1-101) with a molecular weight of 13.6 kDa. This protein features a 20 amino acid His-Tag fused at the N-terminus and undergoes purification using proprietary chromatographic methods.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The S100A7 protein is provided at a concentration of 0.5 mg/ml in a buffer solution containing 20mM Tris-HCl (pH 8), 0.1M NaCl, 1mM DTT, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
S100 calcium-binding protein A7 (psoriasin 1), S100A7c, PSOR1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSNTQAERSI IGMIDMFHKY TRRDDKIDKP SLLTMMKENFPNFLSACDKK GTNYLADVFE KKDKNEDKKI DFSEFLSLLG DIATDYHKQS HGAAPCSGGS Q.

Product Science Overview

Introduction

S100 Calcium Binding Protein A7, also known as Psoriasin, is a member of the S100 family of EF-hand calcium-binding proteins. This protein is encoded by the S100A7 gene, which is located on chromosome 1q21.3 . The recombinant form of this protein, referred to as Human Recombinant S100A7, is produced using E. coli expression systems .

Structure and Function

S100A7 is a small protein with a molecular weight of approximately 11-12 kDa . It contains two EF-hand domains, which are helix-loop-helix structural motifs capable of binding calcium ions . The protein typically forms a noncovalently-linked homodimer .

The primary function of S100A7 is to bind calcium ions, which plays a crucial role in various cellular processes, including cell cycle regulation, cell differentiation, and cell proliferation . It is also involved in the immune response and has been shown to induce the secretion of interleukin-8 (IL-8) in human epithelial carcinoma cells .

Expression and Clinical Relevance

S100A7 was initially identified in the epidermis of psoriatic skin, hence the name Psoriasin . It is highly expressed in psoriatic lesions and is also found in other inflammatory skin conditions. Additionally, S100A7 is expressed in various epithelial tissues and has been associated with several types of cancer, including breast cancer and bladder squamous cell carcinoma .

The overexpression of S100A7 in cancerous tissues suggests that it may play a role in tumorigenesis and cancer progression. It is thought to contribute to the abnormal pathways of differentiation observed in malignant cells .

Recombinant Production

The recombinant form of S100A7 is produced using E. coli expression systems. The protein is typically purified to a high degree of purity (>90% or >95%) using conventional chromatography techniques . The recombinant protein may be tagged with a His-tag at the N-terminus to facilitate purification .

Recombinant S100A7 is used in various research applications, including studies on its role in inflammation, cancer, and other diseases. It is also used to investigate the mechanisms of calcium binding and the regulation of cellular processes by calcium-binding proteins.

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