S100A3 Mouse

S100 Calcium Binding Protein A3 Mouse Recombinant
Cat. No.
BT29964
Source
Escherichia Coli.
Synonyms
Protein S100-A3, Protein S-100E, S100 calcium-binding protein A3, S100a3, S100E.
Appearance
S100A3 is supplied as a sterile filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

S100A3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 125 amino acids (1-101 a.a) and having a molecular mass of 14.3kDa.
S100A3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
S100A3 is a member of the S100 protein family, which are characterized by their two EF-hand calcium-binding motifs. These proteins are typically found in the cytoplasm and nucleus of a wide variety of cells and play a crucial role in regulating various cellular processes, including cell cycle progression and differentiation. Among the S100 proteins, S100A3 stands out with the highest number of cysteines. It exhibits a strong affinity for Zinc and is predominantly found in the human hair cuticle.
Description
Recombinant S100A3 Mouse is produced in E. coli and is a single, non-glycosylated polypeptide chain composed of 125 amino acids (specifically, amino acids 1-101). The protein has a molecular mass of 14.3 kDa. It is engineered with a 24 amino acid His-tag at the N-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.
Physical Appearance
S100A3 is provided as a clear, sterile-filtered solution.
Formulation
The S100A3 protein solution has a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, 2 mM DTT, and 150 mM NaCl.
Stability
For short-term storage (up to 2-4 weeks), S100A3 should be kept at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To ensure long-term stability, adding a carrier protein like HSA or BSA (0.1%) is advisable. It's important to minimize freeze-thaw cycles.
Purity
The purity of S100A3 is determined to be greater than 95.0% using SDS-PAGE analysis.
Synonyms
Protein S100-A3, Protein S-100E, S100 calcium-binding protein A3, S100a3, S100E.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMTRPLE QAVAAIVCTF QEYAGRCGDK YKICQSELKE LLQKELPTWT PSEFRECDYN KFMSVLDTNK DCEVDFGEYV RSLASLCLYC HEYFKECPPE PPCPQ.

Product Science Overview

Introduction

S100 Calcium Binding Protein A3 (S100A3) is a member of the S100 family of proteins, which are characterized by their ability to bind calcium ions through EF-hand motifs. These proteins play crucial roles in various cellular processes, including cell cycle progression, differentiation, and intracellular signaling. S100A3, in particular, is known for its high affinity for both calcium and zinc ions and is predominantly expressed in the hair cuticle.

Structure and Expression

S100A3 is a small protein consisting of 101 amino acids. It contains two EF-hand calcium-binding motifs, which are helix-loop-helix structures that coordinate the binding of calcium ions. The recombinant form of S100A3, expressed in Escherichia coli, is often tagged with a His-tag at the N-terminus to facilitate purification and detection .

Function and Biological Significance

S100A3 is involved in several important biological functions:

  • Calcium and Zinc Binding: S100A3 has a high affinity for calcium and zinc ions, which is essential for its role in cellular signaling and regulation.
  • Hair Cuticle Formation: S100A3 is highly expressed in the hair cuticle, where it is believed to play a role in the differentiation of cuticle cells and the formation of the hair shaft and cuticular barrier .
  • Citrullination: More than half of the arginine residues in S100A3 undergo citrullination, a post-translational modification carried out by peptidylarginine deiminases (PADs). This modification is thought to promote the tetramerization of S100A3, which may be important for its function in hair cuticle cells .
Research and Applications

Recombinant S100A3 is widely used in research to study its structure, function, and role in various biological processes. It is also used in studies related to hair biology and the development of hair care products. The recombinant protein is typically purified to a high degree of purity (>95%) and is suitable for various applications, including SDS-PAGE and other biochemical assays .

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S100A3 Human
S100 Calcium Binding Protein A3 Human Recombinant
Cat. No.
BT29893
Source
Escherichia Coli.
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