S100A10 Mouse
Description
S100A10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Recombinant Mouse S100 Calcium Binding Protein A10, also known as S100A10 or p11, is a member of the S100 family of proteins. These proteins are characterized by their ability to bind calcium ions through EF-hand motifs, which are helix-loop-helix structural domains . S100A10 is encoded by the S100a10 gene in mice and is involved in various cellular processes, including cell cycle progression and differentiation .
S100A10 is a small protein with a molecular mass of approximately 13.6 kDa . It is composed of 120 amino acids and contains two EF-hand calcium-binding motifs . Unlike other S100 proteins, S100A10 does not bind calcium directly but forms a heterotetramer with Annexin A2 (ANXA2), which is essential for its function . This complex plays a crucial role in the regulation of membrane-related processes such as exocytosis and endocytosis .
S100A10 is primarily localized in the cytoplasm and nucleus of various cell types . It is involved in the regulation of several cellular processes, including:
- Cell Cycle Progression: S100A10 influences the progression of the cell cycle by interacting with other proteins involved in cell division .
- Differentiation: It plays a role in the differentiation of cells by modulating the activity of specific transcription factors .
- Membrane Dynamics: The S100A10-ANXA2 complex is crucial for the reorganization of the actin cytoskeleton, which is necessary for vesicle trafficking and membrane repair .
Recombinant S100A10 is typically produced using bacterial expression systems such as Escherichia coli (E. coli) . The gene encoding S100A10 is cloned into an expression vector, which is then introduced into E. coli cells. The bacteria are cultured, and the protein is expressed and purified using chromatographic techniques . The recombinant protein often includes a His-tag to facilitate purification .
Recombinant S100A10 is used in various research applications, including:
- Protein-Protein Interaction Studies: Understanding the interactions between S100A10 and other proteins can provide insights into its biological functions .
- Drug Development: Given its role in mood regulation and neurotransmitter transport, S100A10 is a potential target for drug therapy in mood disorders .
- Cell Biology Research: Studying the effects of S100A10 on cell cycle progression and differentiation can help elucidate the mechanisms underlying these processes .
