RUVBL1 Human
RuvB-Like 1 Human Recombinant
Cat. No.
BT13826
Source
Escherichia Coli.
Synonyms
ECP54, RUVBL1, INO80H, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A, RuvB-Like 1, EC=3.6.4.12, TIP60-associated protein 54-alpha, TAP54-alpha.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RUVBL1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 476 amino acids (1-456 a.a) and having a molecular mass of 52.3kDa. The RUVBL1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
RUVBL1, a component of the NuA4 histone acetyltransferase complex, plays a crucial role in activating specific genes. This activation primarily occurs through the acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is essential for initiating transcriptional programs linked to oncogene and proto-oncogene-driven growth induction, as well as tumor suppressor-mediated growth arrest.
Description
Recombinant Human RUVBL1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 476 amino acids (specifically, amino acids 1 to 456). It has a molecular weight of 52.3 kDa. The RUVBL1 protein is engineered with a 20-amino acid His-Tag at the N-terminus to facilitate purification using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The RUVBL1 protein solution has a concentration of 0.5 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8), 5 mM DTT, 0.2 M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to store the product frozen at -20°C. To further enhance long-term stability, consider adding a carrier protein like HSA or BSA (0.1%). Repeated freezing and thawing of the product should be avoided.
Purity
The purity of RUVBL1 is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
ECP54, RUVBL1, INO80H, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A, RuvB-Like 1, EC=3.6.4.12, TIP60-associated protein 54-alpha, TAP54-alpha.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MKIEEVKSTT KTQRIASHSH VKGLGLDESG LAKQAASGLV GQENAREACG VIVELIKSKK MAGRAVLLAG PPGTGKTALA LAIAQELGSK VPFCPMVGSE VYSTEIKKTE VLMENFRRAI GLRIKETKEV YEGEVTELTP CETENPMGGY GKTISHVIIG LKTAKGTKQL KLDPSIFESL QKERVEAGDV IYIEANSGAV KRQGRCDTYA TEFDLEAEEY VPLPKGDVHK KKEIIQDVTL HDLDVANARP QGGQDILSMM GQLMKPKKTE ITDKLRGEIN KVVNKYIDQG IAELVPGVLF VDEVHMLDIE CFTYLHRALE SSIAPIVIFA SNRGNCVIRG
TEDITSPHGI PLDLLDRVMI IRTMLYTPQE MKQIIKIRAQ TEGINISEEA LNHLGEIGTK TTLRYSVQLL TPANLLAKIN GKDSIEKEHV
EEISELFYDA KSSAKILADQ QDKYMK.
TEDITSPHGI PLDLLDRVMI IRTMLYTPQE MKQIIKIRAQ TEGINISEEA LNHLGEIGTK TTLRYSVQLL TPANLLAKIN GKDSIEKEHV
EEISELFYDA KSSAKILADQ QDKYMK.
Product Science Overview
Structure and Function
RUVBL1 is a component of several multi-protein complexes, such as the NuA4 histone acetyltransferase complex and the INO80 chromatin remodeling complex . It possesses ATPase and DNA helicase activities, which are essential for its role in chromatin remodeling and transcription regulation . The protein can form hexamers, which further assemble into dodecamers with another protein, RUVBL2 . This hexamerization is crucial for its ATP hydrolysis activity .
Biological Roles
- Chromatin Remodeling: RUVBL1 is a part of the NuA4 complex, which acetylates histones H4 and H2A, leading to transcriptional activation of specific genes .
- Transcription Regulation: It modulates transcriptional activation by interacting with transcription factors such as MYC and the LEF1/TCF1-CTNNB1 complex .
- DNA Repair: RUVBL1 is involved in DNA recombination and repair processes, contributing to the maintenance of genomic stability .
- Cell Proliferation: It plays a critical role in cell division and proliferation, making it essential for cellular growth and development .
Expression and Localization
Recombinant RUVBL1
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