RUVBL1 Antibody
Description
Product Specs
Product Science Overview
RuvB-Like 1 (RUVBL1), also known as TIP49, Pontin52, and INO80H, is a protein encoded by the RUVBL1 gene in humans . This protein is a member of the AAA+ (ATPases Associated with diverse cellular Activities) protein family, which is characterized by its ATPase and DNA helicase activities . RUVBL1 plays a crucial role in various cellular processes, including transcriptional regulation, chromatin remodeling, and DNA repair .
RUVBL1 can form hexamers, which further assemble into dodecamers with another protein, RUVBL2 . This structural arrangement is essential for its ATPase activity and its role in chromatin remodeling complexes such as the INO80 and TIP60 complexes . These complexes are involved in transcriptional activation by acetylating nucleosomal histones H4 and H2A, thereby facilitating access to DNA for transcription factors .
RUVBL1 is implicated in several critical biological pathways, including transcription-coupled nucleotide excision repair (TC-NER) and chromatin organization . It is also associated with various diseases, such as common variable immunodeficiency and isolated polycystic liver disease . The protein’s ability to hydrolyze ATP and unwind DNA makes it a vital component in maintaining genomic stability and regulating gene expression .
The mouse anti-human RUVBL1 antibody is a monoclonal antibody that specifically recognizes the RuvB-like 1 protein in humans . This antibody is commonly used in research to study the expression and function of RUVBL1 in various biological contexts. It is particularly useful in techniques such as Western blotting, where it detects a band of approximately 50 kDa corresponding to the RUVBL1 protein .
The mouse anti-human RUVBL1 antibody is prepared by immunizing mice with a recombinant human RUVBL1 protein, followed by purification using affinity chromatography . This antibody is available in purified form and is typically stored in phosphate-buffered saline with preservatives to maintain its stability . Researchers use this antibody to investigate the role of RUVBL1 in cellular processes, including its involvement in chromatin remodeling and transcriptional regulation .
