RPL35A Human

Ribosomal Protein L35A (RPL35A) is a crucial component of the large 60S subunit of eukaryotic ribosomes. Ribosomes are essential organelles responsible for protein synthesis in cells. The RPL35A gene encodes a protein that is part of the ribosomal machinery, playing a significant role in the translation process.

The RPL35A gene is located on chromosome 3q29-qter . It encodes a protein that belongs to the L35AE family of ribosomal proteins. The human recombinant RPL35A protein is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 133 amino acids, with a molecular mass of approximately 14.9 kDa . The recombinant protein is often fused to a His-tag at the N-terminus to facilitate purification.

RPL35A is a component of the large ribosomal subunit and is involved in the synthesis of proteins by translating messenger RNA (mRNA) into polypeptide chains . It binds to both initiator and elongator transfer RNAs (tRNAs) and is located at the P site, or P and A sites, of the ribosome . This positioning is crucial for the accurate translation of genetic information.

Mutations in the RPL35A gene have been associated with Diamond-Blackfan Anemia (DBA), a rare congenital disorder characterized by failure of the bone marrow to produce red blood cells . Patients with DBA often present with anemia, physical abnormalities, and an increased risk of cancer. The study of RPL35A and its role in ribosome function is essential for understanding the molecular mechanisms underlying DBA and developing potential therapeutic strategies.

The recombinant form of RPL35A is used in various research applications, including studies on ribosome structure and function, protein synthesis, and the development of therapeutic interventions for ribosomopathies. The availability of human recombinant RPL35A allows researchers to investigate its interactions with other ribosomal proteins and nucleic acids, providing insights into the complex dynamics of ribosome assembly and function.