RPL34 Human

Ribosomal Protein L34 Human Recombinant
Cat. No.
BT16421
Source
Escherichia Coli.
Synonyms
Ribosomal Protein L34, 60S Ribosomal Protein L34, Leukemia-Associated Protein, L34.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RPL34 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (1-117) and having a molecular mass of 15.7kDa.
RPL34 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RPL34, a member of the L34E family of ribosomal proteins, is located within the 60S subunit of ribosomes and plays a crucial role in protein synthesis. Ribosomes, responsible for catalyzing protein synthesis, consist of a small 40S subunit and a large 60S subunit, composed of 4 RNA species and approximately 80 distinct proteins. Initially mapped to chromosome 17q21, RPL34 is now known to reside on chromosome 4q. The gene encoding RPL34 undergoes alternative splicing, transcription initiation, and polyadenylation, resulting in various transcript variants that ultimately produce the same protein. Notably, as with many genes encoding ribosomal proteins, multiple processed pseudogenes of RPL34 are dispersed throughout the genome.
Description
Recombinant human RPL34, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 15.7 kDa. The protein consists of 140 amino acids, with amino acids 1-117 corresponding to the RPL34 sequence. A 23 amino acid His-tag is fused to the N-terminus to facilitate purification. The protein has been purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The RPL34 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the RPL34 solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To further enhance long-term stability during frozen storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of RPL34 is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Ribosomal Protein L34, 60S Ribosomal Protein L34, Leukemia-Associated Protein, L34.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVQRLTY RRRLSYNTAS NKTRLSRTPG NRIVYLYTKK VGKAPKSACG VCPGRLRGVR AVRPKVLMRL SKTKKHVSRA YGGSMCAKCV RDRIKRAFLI EEQKIVVKVL KAQAQSQKAK

Product Science Overview

Introduction

Ribosomal Protein L34 (RPL34) is a highly conserved protein that is a component of the 60S large subunit of mammalian ribosomes. Ribosomes are essential organelles responsible for protein synthesis in cells. The 60S subunit, along with the 40S subunit, forms the complete ribosome. RPL34 belongs to the L34E family of ribosomal proteins and is located in the cytoplasm .

Gene and Protein Structure

The RPL34 gene is located on chromosome 4q, although it was initially thought to be located at 17q21 . The gene encodes a protein that is part of the large ribosomal subunit. The protein is involved in the structural stability of ribosomal RNA (rRNA) and the accurate folding of rRNA during ribosomal assembly . Alternative splicing of the RPL34 gene results in multiple transcript variants, all encoding the same isoform .

Biological Function

RPL34 plays a crucial role in the synthesis of proteins by facilitating the translation of mRNA into polypeptides. It is a structural constituent of the ribosome and is involved in RNA binding . The protein’s function is essential for maintaining the integrity and function of the ribosome, which is critical for cellular protein synthesis.

Clinical Significance

Overexpression of RPL34 has been observed in various cancer cells, indicating its potential role in tumorigenesis . For example, RPL34 has been found to be dysregulated in hilar cholangiocarcinoma (HCCA), a type of liver cancer. High expression levels of RPL34 in HCCA are associated with poor clinical outcomes, including shorter time to recurrence and lower survival rates . Silencing RPL34 expression in cancer cells has been shown to inhibit cell proliferation and migration, suggesting that RPL34 could be a potential therapeutic target for cancer treatment .

Research and Applications

Research on RPL34 has focused on its role in cancer biology and its potential as a prognostic biomarker and therapeutic target. Studies have demonstrated that targeting RPL34 can attenuate tumor growth and improve clinical outcomes in cancer patients . Additionally, recombinant RPL34 protein is used in various research applications to study ribosome function and protein synthesis.

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