RPL11 Human
Ribosomal Protein L11 Human Recombinant
Cat. No.
BT15758
Source
Escherichia Coli.
Synonyms
Ribosomal Protein L11, Cell Growth-Inhibiting Protein 34, CLL-Associated Antigen KW-12, 60S Ribosomal Protein L11, DBA7, GIG34, L11.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RPL11 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-178) and having a molecular mass of 22.6kDa.
RPL11 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
RPL11 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Ribosomes, the protein synthesis machinery within cells, consist of a small 40S subunit and a larger 60S subunit. Ribosomal Protein L11 (RPL11), a member of the L5P family, is an integral part of the 60S subunit. This protein, located within the cytoplasm, is believed to interact with the 5S rRNA molecule.
Description
Recombinant human RPL11, expressed in E.coli, is a single, non-glycosylated polypeptide chain. It comprises 201 amino acids, including a 23 amino acid His-tag at the N-terminus, and has a molecular weight of 22.6kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized through filtration.
Formulation
The RPL11 solution is provided at a concentration of 0.5mg/ml. It is buffered with 20mM Tris-HCl (pH 8.0) and contains 0.2M NaCl, 1mM DTT, 2mM EDTA, 250mM Imidazole, and 50% glycerol.
Stability
For short-term storage (up to 4 weeks), the solution should be kept refrigerated at 4°C. For longer periods, it is recommended to store the protein frozen at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Analysis by SDS-PAGE confirms a purity level exceeding 90%.
Synonyms
Ribosomal Protein L11, Cell Growth-Inhibiting Protein 34, CLL-Associated Antigen KW-12, 60S Ribosomal Protein L11, DBA7, GIG34, L11.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQDQGE KENPMRELRI RKLCLNICVG ESGDRLTRAA KVLEQLTGQT PVFSKARYTV RSFGIRRNEK IAVHCTVRGA KAEEILEKGL KVREYELRKN NFSDTGNFGF GIQEHIDLGI KYDPSIGIYG LDFYVVLGRP GFSIADKKRR TGCIGAKHRI SKEEAMRWFQ QKYDGIILPG K
Product Science Overview
Gene and Protein Structure
Function
Ribosomes are essential organelles that catalyze protein synthesis. They consist of a small 40S subunit and a large 60S subunit. RPL11 is a component of the 60S subunit and is involved in the assembly and function of the ribosome . It plays a role in the translation of mRNA into proteins, a fundamental process for cell growth and function.
Interactions
RPL11 interacts with several other proteins and molecules within the cell. Notably, it has been shown to interact with:
- MDM2: RPL11 negatively regulates the oncoprotein MDM2, which is involved in the p53-dependent ribosomal-stress checkpoint pathway .
- NOP53: Another interaction partner, which is involved in ribosome biogenesis .
- P53: A well-known tumor suppressor protein, indicating RPL11’s role in cellular stress responses .
Clinical Significance
Alternative Splicing and Pseudogenes
Research and Applications
Human recombinant RPL11 is used in various research applications to study its function and interactions. It is also valuable in understanding ribosome biogenesis and the molecular mechanisms underlying diseases associated with ribosomal dysfunction.
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