RNF7 Human

Ring Finger Protein 7 Human Recombinant
Cat. No.
BT18679
Source
Escherichia Coli.
Synonyms
RING-box protein 2 isoform 1, Ring finger protein 7, CKBBP1, ROC2, SAG, RING-box protein 2, Rbx2, CKII beta-binding protein 1, Regulator of cullins 2, Sensitive to apoptosis gene protein, RBX2, RNF7.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RNF7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 136 amino acids (1-113 a.a) and having a molecular mass of 15.1kDa.
RNF7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ring Finger Protein 7, also known as RNF7, is a highly conserved protein characterized by the presence of a ring finger domain. This protein plays a crucial role as a component of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are integral to the protein degradation machinery. These ligases are essential for regulating cell cycle progression and signal transduction pathways. RNF7 is known to be a substrate of casein kinase II (CSNK2A1/CKII) and also interacts with it. Notably, the phosphorylation of RNF7 by CSNK2A1 facilitates the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1 (CDKN1B).
Description
This product consists of the recombinant human RNF7 protein, produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 136 amino acids (specifically, amino acids 1 through 113). The protein has a molecular weight of 15.1 kDa. For purification and detection purposes, the RNF7 protein is fused to a 23 amino acid His-tag at its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The RNF7 protein is supplied in a solution at a concentration of 1 mg/ml. The solution buffer consists of 20mM Tris-HCl at pH 8.0, 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For longer storage periods, it is recommended to store the product in a frozen state at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised. It's important to avoid repeated cycles of freezing and thawing the product.
Purity
The purity of the RNF7 protein in this product is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
RING-box protein 2 isoform 1, Ring finger protein 7, CKBBP1, ROC2, SAG, RING-box protein 2, Rbx2, CKII beta-binding protein 1, Regulator of cullins 2, Sensitive to apoptosis gene protein, RBX2, RNF7.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADVEDG EETCALASHS GSSGSKSGGD KMFSLKKWNA VAMWSWDVEC DTCAICRVQV MDACLRCQAE NKQEDCVVVW GECNHSFHNC CMSLWVKQNN RCPLCQQDWV VQRIGK.

Product Science Overview

Introduction

Ring Finger Protein 7 (RNF7), also known as Sensitive to Apoptosis Gene (SAG), is a highly conserved protein that functions as an E3 ubiquitin ligase. This protein plays a crucial role in the ubiquitination process, which is a post-translational modification that regulates the degradation of proteins within the cell. RNF7 is involved in various cellular processes, including cell cycle regulation, apoptosis, and signal transduction.

Structure and Function

RNF7 contains a RING (Really Interesting New Gene) finger domain, which is essential for its function as an E3 ubiquitin ligase. The RING domain facilitates the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to the target substrate, marking it for degradation by the proteasome. This process is vital for maintaining cellular homeostasis and regulating protein levels within the cell .

Role in Cancer

Overexpression of RNF7 has been observed in multiple human cancers, including renal cell carcinoma (RCC). In RCC, RNF7 inhibits apoptosis and promotes glycolysis, contributing to tumor growth and progression. The elevated expression of RNF7 in tumor tissues is correlated with poor survival outcomes in patients. RNF7 achieves these effects by activating the JAK/STAT3 signaling pathway through the ubiquitination of SOCS1 (Suppressor of Cytokine Signaling 1) .

Mechanisms of Action

RNF7’s role in cancer is primarily mediated through its function as an E3 ubiquitin ligase. By ubiquitinating specific target proteins, RNF7 can regulate various signaling pathways that control cell proliferation, apoptosis, and metabolism. For example, in RCC, RNF7 promotes tumor growth by inhibiting apoptosis and enhancing glycolysis. This is achieved through the activation of the JAK/STAT3 signaling pathway, which is a critical regulator of cell survival and proliferation .

Therapeutic Potential

Given its role in cancer progression, RNF7 represents a potential therapeutic target for cancer treatment. Inhibiting RNF7’s activity could restore normal cellular processes and reduce tumor growth. Research is ongoing to develop specific inhibitors that can target RNF7 and disrupt its function in cancer cells .

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THE BIOTEK
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