RNF181 Human
Description
RNF181 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Ring Finger Protein 181 (RNF181) is a protein encoded by the RNF181 gene in humans. This protein is part of the E3 ubiquitin-protein ligase family, which plays a crucial role in the ubiquitination process. Ubiquitination is a post-translational modification where ubiquitin, a small regulatory protein, is attached to a substrate protein. This process is essential for various cellular functions, including protein degradation, DNA repair, and cell cycle regulation.
RNF181 is characterized by the presence of a RING (Really Interesting New Gene) finger domain, which is a specialized type of zinc finger domain. This domain is responsible for the protein’s E3 ubiquitin ligase activity. RNF181 accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates .
The protein is widely expressed in various tissues, with the highest levels found in the liver and heart, and the lowest levels in the brain and skeletal muscle . RNF181 is also expressed in platelets at the protein level .
The human recombinant RNF181 protein is typically produced using bacterial expression systems, such as Escherichia coli (E. coli). The recombinant protein is expressed as a single polypeptide chain containing 176 amino acids, with a molecular mass of approximately 20.3 kDa . The protein is often fused to a His-tag at the N-terminus to facilitate purification using chromatographic techniques .
The purified RNF181 protein is formulated in a buffer containing Tris-HCl, NaCl, DTT, and glycerol to ensure stability and prevent degradation . The protein is stored at 4°C for short-term use or frozen at -20°C for long-term storage .
RNF181 plays a significant role in the ubiquitin-proteasome system, which is responsible for the degradation of misfolded or damaged proteins. By tagging these proteins with ubiquitin, RNF181 facilitates their recognition and degradation by the 26S proteasome . This process is crucial for maintaining cellular homeostasis and preventing the accumulation of toxic protein aggregates.
Additionally, RNF181 has been implicated in various cellular pathways, including protein ubiquitination and metabolism of proteins . The protein’s E3 ubiquitin ligase activity is essential for regulating the stability and function of its substrate proteins, thereby influencing numerous cellular processes.
