RND1 Human

Rho Family GTPase 1 Human Recombinant
Cat. No.
BT15272
Source
E.coli.
Synonyms
Rho family GTPase 1, GTP-binding protein, ras homolog gene family, member S, ARHS, RHOS, rho-related GTP-binding protein Rho6, RHO6, Rnd1, FLJ42294.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RND1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 220 amino acids (1-200) and having a molecular mass of 24.5 kDa.
The RND1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
RND1, a member of the Rho family of GTPases, forms a distinct branch due to its unique charge, size, and biochemical properties. Existing primarily in an activated, GTP-bound state, RND1 negatively regulates both actin assembly and cell adhesion. It plays a crucial role in modulating the organization of the actin cytoskeleton in response to extracellular growth factors.
Description
Recombinant Human RND1, expressed in E.coli, is a single, non-glycosylated polypeptide chain consisting of 220 amino acids (residues 1-200). It has a molecular weight of 24.5 kDa. The RND1 protein is fused to a 20 amino acid His-Tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The RND1 solution is provided at a concentration of 0.5 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 0.1 M NaCl, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like 0.1% HSA or BSA is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the RND1 protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Rho family GTPase 1, GTP-binding protein, ras homolog gene family, member S, ARHS, RHOS, rho-related GTP-binding protein Rho6, RHO6, Rnd1, FLJ42294.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MKERRAPQPV VARCKLVLVG DVQCGKTAML QVLAKDCYPE TYVPTVFENY TACLETEEQR VELSLWDTSG SPYYDNVRPL CYSDSDAVLL CFDISRPETV DSALKKWRTE ILDYCPSTRV LLIGCKTDLR TDLSTLMELS HQKQAPISYE QGCAIAKQLG AEIYLEGSAF TSEKSIHSIF RTASMLCLNK PSPLPQKSPV

Product Science Overview

Introduction

The Rho family of GTPases is a subfamily of the Ras superfamily of small (~21 kDa) signaling G proteins. These proteins play a crucial role in regulating various aspects of intracellular actin dynamics, which are essential for cell movement, organelle development, and other cellular functions . The Rho family includes several members, with RhoA, Rac1, and Cdc42 being the most extensively studied .

Discovery and Evolution

The identification of the Rho family of GTPases began in the mid-1980s. The first member, RhoA, was isolated in 1985 through a low stringency cDNA screening . Subsequently, Rac1 and Rac2 were identified in 1989, followed by Cdc42 in 1990 . Over the years, additional members were discovered, leading to the identification of 20 mammalian Rho GTPases distributed across eight subfamilies .

Structure and Function

Rho GTPases act as molecular switches, cycling between an inactive GDP-bound state and an active GTP-bound state . This switching mechanism allows them to interact with various downstream effectors, thereby regulating multiple cellular processes. The primary functions of Rho GTPases include:

  • Cytoskeletal Dynamics: Rho GTPases regulate the organization of the actin cytoskeleton, influencing cell shape, polarity, and movement .
  • Cell Division and Proliferation: They play a role in cell cycle progression and mitosis .
  • Gene Expression: Rho GTPases are involved in the regulation of gene transcription .
  • Cell Adhesion: They modulate cell-cell and cell-matrix adhesion, which are critical for tissue integrity and repair .
Mechanisms of Regulation

The activity of Rho GTPases is tightly regulated by three classes of proteins:

  1. Guanine Nucleotide Exchange Factors (GEFs): These proteins activate Rho GTPases by facilitating the exchange of GDP for GTP .
  2. GTPase-Activating Proteins (GAPs): GAPs inactivate Rho GTPases by accelerating the hydrolysis of GTP to GDP .
  3. Guanine Nucleotide Dissociation Inhibitors (GDIs): GDIs sequester Rho GTPases in the cytoplasm, preventing their activation .
Human Recombinant Rho GTPase 1

Human recombinant Rho GTPase 1 is a laboratory-produced version of the naturally occurring protein. It is used extensively in research to study the protein’s structure, function, and role in various cellular processes. Recombinant proteins are produced by inserting the gene encoding the protein into an expression system, such as bacteria or yeast, which then synthesizes the protein in large quantities.

Applications in Research

Recombinant Rho GTPase 1 is used in various experimental setups to:

  • Investigate Signal Transduction Pathways: Understanding how Rho GTPases transmit signals within cells .
  • Study Cytoskeletal Dynamics: Exploring the role of Rho GTPases in actin filament organization .
  • Examine Disease Mechanisms: Researching the involvement of Rho GTPases in diseases such as cancer and neurodegenerative disorders .

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