RLN2 Human

Relaxin-2 Human Recombinant
Cat. No.
BT13377
Source
Escherichia Coli.
Synonyms
Prorelaxin H2, RLN2, H2, RLXH2, H2-RLX, bA12D24.1.1, bA12D24.1.2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RLN2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids (25-185 a.a) and having a molecular mass of 20.7kDa.
RLN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Prorelaxin H2 (RLN2), a member of a hormone family primarily produced by the ovary, plays a crucial role in the mammalian reproductive system. It facilitates cervical ripening, elongates the pubic symphysis, and inhibits uterine contractions. Beyond its reproductive functions, RLN2 may contribute to sperm motility, blood pressure regulation, heart rate control, and vasopressin release. This peptide hormone is associated with various therapeutically significant physiological effects, including collagen metabolism regulation and involvement in multiple vascular control pathways. The active form of RLN2 comprises an A chain and a B chain connected by disulfide bonds.
Description
Recombinant human RLN2, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 184 amino acids (25-185 a.a). With a molecular mass of 20.7 kDa, this protein is fused to a 23 amino acid His-tag at its N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution.
Formulation
The RLN2 protein solution (1 mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0), containing 0.4M Urea and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
Prorelaxin H2, RLN2, H2, RLXH2, H2-RLX, bA12D24.1.1, bA12D24.1.2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSDSWMEEV IKLCGRELVR AQIAICGMST WSKRSLSQED APQTPRPVAE IVPSFINKDT ETINMMSEFV ANLPQELKLT LSEMQPALPQ LQQHVPVLKD SSLLFEEFKK LIRNRQSEAA DSSPSELKYL GLDTHSRKKR QLYSALANKC CHVGCTKRSL ARFC.

Product Science Overview

Discovery and Structure

Relaxin-2 was first discovered in 1926 by Frederick Hisaw . It is a 6 kDa polypeptide hormone consisting of 48 amino acids . Relaxin-2 is structurally similar to insulin and is the only relaxin that circulates in the blood . The hormone is a heterodimer composed of two peptide chains, A and B, linked by disulfide bonds .

Functions

Relaxin-2 plays a significant role in female reproduction. It is involved in:

  • Lengthening of the pubic symphysis and softening of the tissues of the birth canal .
  • Stimulation of the mammary gland and endometrial development .
  • Maintenance of myometrial quiescence during pregnancy .

Additionally, relaxin-2 has been found to enhance sperm motility, regulate blood pressure, control heart rate, and release oxytocin and vasopressin .

Preparation Methods

Recombinant human relaxin-2 is typically produced using Escherichia coli (E. coli) expression systems . The recombinant protein is purified to achieve a high level of purity, often greater than 97%, as determined by SDS-PAGE under reducing conditions . The endotoxin level is kept below 0.01 EU per 1 μg of the protein by the Limulus Amebocyte Lysate (LAL) method .

Applications

Recombinant human relaxin-2 has several applications in research and medicine:

  • Drug Discovery and Development: It is used as an agonist for RXFP1 (LGR7) and RXFP2 (LGR8) receptors .
  • Cardiovascular Research: Studies have shown that relaxin-2 can protect cardiac cells and improve heart function .
  • Reproductive Health: It is used to study its effects on female reproductive tissues and processes .

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