Resistin Mouse, Flag
Description
Product Specs
Resistin, encoded by the RSTN gene, is a peptide hormone classified as a cysteine-rich secreted protein within the RELM family. It is also known as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin, initially a 108-amino acid prepeptide, undergoes cleavage of its hydrophobic signal peptide before secretion. In human blood, it circulates as a dimer, formed by two 92-amino acid polypeptides linked by a disulfide bond at Cys26.
Resistin is suggested to play a role in obesity resistance. Studies indicate that mouse resistin, primarily produced and secreted by adipocytes, can decrease the sensitivity of skeletal muscle myocytes, hepatocytes, and adipocytes to insulin. Elevated resistin levels have been observed in obese mice, with levels decreasing during fasting and upon administration of antidiabetic drugs. Conversely, Way et al. reported that resistin expression is significantly reduced in obesity and upregulated by certain antidiabetic drugs.
Additional research suggests that mouse resistin levels increase during adipocyte differentiation while simultaneously inhibiting the process. In contrast, human adipogenic differentiation appears to correlate with downregulation of resistin gene expression.
Product Science Overview
Resistin is a member of the resistin-like molecule (RELM) family, which includes RELMα, RELMβ, and RELMγ. The structure of resistin comprises a carboxy-terminal disulfide-rich β-sandwich “head” domain and an amino-terminal α-helical “tail” segment . In mice, resistin is primarily expressed in adipocytes and has been implicated in insulin resistance and type 2 diabetes .
Recombinant resistin is produced using Escherichia coli (E. coli) expression systems. The recombinant protein contains 93 amino acid residues of mouse resistin and 31 additional amino acid residues, including a signal sequence of phage fd and a C-terminal FLAG tag . The FLAG tag is an artificial peptide sequence (DYKDDDDK) used for protein purification and detection .
The FLAG tag is a widely used epitope tag for protein purification and detection. It is highly specific and can be used in various assays, including immunoprecipitation, Western blotting, and immunofluorescence . The FLAG tag’s small size and hydrophilic nature make it less likely to interfere with the protein’s function .
Resistin has been studied extensively for its role in metabolic and inflammatory diseases. In mice, resistin is associated with obesity and insulin resistance, while in humans, it is considered a pro-inflammatory molecule expressed in immune cells . Resistin’s involvement in chronic inflammatory diseases, metabolic disorders, and cancers highlights its importance as a potential therapeutic target .
Recombinant mouse resistin with a FLAG tag is used in various research applications, including:
- Studying insulin resistance: Investigating the molecular mechanisms underlying insulin resistance and type 2 diabetes.
- Inflammatory response: Exploring resistin’s role in chronic inflammatory diseases and immune response modulation.
- Protein-protein interactions: Using the FLAG tag for affinity purification and studying interactions with other proteins.
