Resistin Human, His
Description
The Resistin is purified by proprietary chromatographic techniques.
Product Specs
Resistin is implicated as a potential link between obesity and insulin resistance. Studies suggest that mouse resistin, primarily produced and secreted by adipocytes, can decrease insulin sensitivity in skeletal muscle myocytes, hepatocytes, and adipocytes. Steppan et al. proposed that resistin inhibits insulin's ability to stimulate glucose uptake and observed elevated resistin levels in obese mice, which were reduced by fasting and antidiabetic drugs. Conversely, Way et al. reported that resistin expression is significantly suppressed in obesity and upregulated by certain antidiabetic drugs.
Research indicates that mouse resistin levels increase during adipocyte differentiation but may also have an inhibitory effect on adipogenesis. In contrast, human adipogenic differentiation appears to be linked to a downregulation of resistin gene expression.
The purification of Resistin is achieved through proprietary chromatographic techniques.
Repeated freezing and thawing should be avoided.
Product Science Overview
Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1), is a cysteine-rich peptide hormone derived from adipose tissue. In humans, it is encoded by the RETN gene . Resistin has been a subject of extensive research due to its controversial role in obesity, insulin resistance, and inflammation.
Resistin is a small protein with a molecular weight of approximately 12.5 kDa in humans. It consists of 108 amino acid residues in humans and 114 in mice and rats . The structure of resistin mainly comprises a carboxy-terminal disulfide-rich β-sandwich “head” domain with positive electrostatic surfaces and an amino-terminal α-helical “tail” segment with negative electrostatic potential . The crystal structure reveals a multimeric assembly consisting of hexamer-forming disulfide bonds .
Resistin was initially proposed as a link between obesity and diabetes in mice. In humans, resistin is considered to be a pro-inflammatory molecule expressed in immune cells, playing a regulatory role in many chronic inflammatory diseases, metabolic diseases, infectious diseases, and cancers . It has been shown to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice, the levels of serum resistin are increased, which has an antagonistic effect on insulin .
Human recombinant resistin is produced in E. coli as a single, non-glycosylated polypeptide chain containing a 92 amino acids fragment (17-108) of the mature human resistin. This recombinant protein is fused with a 4.5 kDa amino-terminal hexahistidine tag (His Tag) and purified by proprietary chromatographic techniques . The His Tag facilitates purification and detection of the protein.
Resistin has several applications in research and clinical settings. It is used as a biomarker for various diseases, including diabetes, obesity, cardiovascular diseases, and atherosclerosis . Additionally, resistin has been identified as a potential diagnostic biomarker for sepsis, with elevated levels observed in sepsis patients . Its role in modulating immune responses and inflammation makes it a valuable target for developing new therapeutic agents.
