RELM a Mouse
RELM-alpha Mouse Recombinant
Cat. No.
BT20189
Source
Escherichia Coli.
Synonyms
Resistin-like alpha, RELMalpha, Cysteine-rich secreted protein FIZZ1, Parasite-induced macrophage novel gene 1 protein, Cysteine-rich secreted protein A12-gamma, RELM-a.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98% as determined by SDS-PAGE & RP-HPLC.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Mouse RELM-alpha Recombinant produced in E.Coli is a monomeric, non-glycosylated, polypeptide chain containing 88 amino acids and having a molecular mass of 10 kDa. The Mouse RELM-alpha is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Bronchoalveolar lavage fluid obtained from mice experiencing experimentally induced allergic pulmonary inflammation has been found to contain a previously unidentified 9.4 kDa cysteine-rich secreted protein known as RELM-alpha (FIZZ1, found in inflammatory zone). This protein is primarily found within the stroma of adipose tissue. Murine RELM-alpha (FIZZ1) is recognized as the first member of a newly identified gene family. This family includes two other murine genes, one expressed in intestinal crypt epithelium (RELM-beta) and the other in white adipose tissue (Resistin), as well as two related human genes.
Description
Recombinant Mouse RELM-alpha, produced in E. coli, is a monomeric polypeptide chain that lacks glycosylation. It comprises 88 amino acids, resulting in a molecular weight of 10 kDa. The purification of Mouse RELM-alpha is achieved through proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The product undergoes sterile filtration and lyophilization from a solution of 0.5mg/ml in 10mM sodium phosphate buffer with a pH of 7.5.
Solubility
It can be reconstituted to a concentration of 0.1mg/ml in deionized sterile water.
Stability
For long-term storage, the lyophilized protein should be kept at -20°C. To minimize degradation caused by repeated freezing and thawing, it is recommended to aliquot the product after reconstitution. The reconstituted protein remains stable at 4°C for a limited period; no changes are observed within two weeks of storage at this temperature.
Purity
The purity of the protein is determined to be greater than 98% through SDS-PAGE and RP-HPLC analysis.
Synonyms
Resistin-like alpha, RELMalpha, Cysteine-rich secreted protein FIZZ1, Parasite-induced macrophage novel gene 1 protein, Cysteine-rich secreted protein A12-gamma, RELM-a.
Source
Escherichia Coli.
Amino Acid Sequence
MDETIEIIVE NKVKELLANP ANYPSTVTKT LSCTSVKTMN RWASCPAGMT ATGCACGFAC GSWEIQSGDT CNCLCLLVDW TTARCCQLS.
Product Science Overview
Discovery and Nomenclature
RELM-alpha was first discovered in 2000 by Holcomb et al. in bronchoalveolar lavage fluid from mice with experimentally induced allergic pulmonary inflammation . Due to its discovery in various tissues and diseases, RELM-alpha has several synonyms, including Cysteine-rich secreted protein A12-γ and Parasite-induced macrophage novel gene 1 protein .
Structure and Expression
Biological Functions
RELM-alpha plays a crucial role in various physiological and pathological processes, including:
- Inflammatory Response: It is involved in the regulation of inflammation, particularly in lung and intestinal tissues .
- Cell Proliferation: RELM-alpha influences the proliferation of lung cells .
- Glucose Metabolism: It has a role in glucose metabolism, impacting energy homeostasis .
- Barrier Defense: RELM-alpha contributes to the defense mechanisms of skin and colon barriers .
Signaling Pathways
Clinical Relevance
RELM-alpha is associated with the progression of multiple diseases, such as:
- Lung Diseases: It is implicated in various pulmonary conditions, including asthma and chronic obstructive pulmonary disease (COPD) .
- Intestinal Diseases: RELM-alpha plays a role in inflammatory bowel diseases (IBD) like Crohn’s disease and ulcerative colitis .
- Cardiovascular Diseases: It is involved in the pathogenesis of cardiovascular conditions .
- Cancers: RELM-alpha has been linked to the development and progression of certain cancers .
Recombinant Production
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