RELM a Mouse, His

RELM-Alpha Mouse Recombinant, His Tag
Cat. No.
BT20300
Source
Escherichia Coli.
Synonyms
Resistin-like alpha, RELMalpha, Cysteine-rich secreted protein FIZZ1, Parasite-induced macrophage novel gene 1 protein, Cysteine-rich secreted protein A12-gamma, RELM-a.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RELM-alpha Mouse Recombinant is manufactured with a signal sequence of phage fd (20aa) and C-terminal fusion of flagTag (10aa). The RELM-alpha Flag-Tagged Fusion Protein is a 13.3 kDa protein containing 91 amino acid residues with 30 additional amino acid residues - signal sequence of phage fd, flagTag (underlined).

Product Specs

Introduction
RELM-alpha (FIZZ1, found in inflammatory zone) is a 9.4 kDa cysteine-rich secreted protein initially discovered in the bronchoalveolar lavage fluid of mice experiencing experimentally induced allergic pulmonary inflammation. Although primarily found in adipose tissue stroma, RELM-alpha exhibits a limited tissue distribution. This protein is the foundational member of a gene family that comprises two additional murine genes, RELM-beta (expressed in intestinal crypt epithelium) and Resistin (expressed in white adipose tissue), along with two related human genes. Studies show that RELM-alpha can inhibit the differentiation of 3T3-L1 preadipocytes into adipocytes without impacting their proliferation. Furthermore, it can form heterooligomers with resistin, but not with RELM-beta. These findings, coupled with its expression in adipose tissue and its secreted nature, suggest that RELM-alpha may play a role in adipogenesis and muscle differentiation. In the lungs, RELM-alpha, induced by hypoxia and renamed hypoxia-induced mitogenic factor (HIMF), activates Akt phosphorylation via the phosphatidylinositol 3-kinase (PI3K) pathway, as evidenced by the inhibitory effect of LY294002 (a PI3K inhibitor) on HIMF-induced Akt phosphorylation and RPSM proliferation. These observations indicate the PI3K/Akt pathway's involvement in HIMF's proliferative effects. Further investigations revealed HIMF's angiogenic and vasoconstrictive properties, demonstrated by its ability to elevate pulmonary arterial pressure and vascular resistance. Additionally, studies suggest that HIMF may regulate apoptosis and contribute to lung alveolarization and maturation.
Description
Recombinant Mouse RELM-alpha is designed with a phage fd signal sequence (20aa) and a C-terminal fusion of flagTag (10aa). The resulting RELM-alpha Flag-Tagged Fusion Protein has a molecular weight of 13.3 kDa and comprises 91 amino acid residues of the target protein along with 30 additional amino acid residues from the phage fd signal sequence and the flagTag (underlined).
Physical Appearance
White lyophilized powder after filtration.
Formulation
Lyophilized from a 0.5 mg/ml solution in 5mM Tris pH 7.5, 25mM NaCl, and filtered.
Solubility

Reconstitute the lyophilized pellet completely by adding deionized water.

Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize repeated freeze-thaw cycles. The reconstituted protein remains stable at 4°C for a limited period and shows no significant change for up to two weeks.
Purity
Purity determined by SDS-PAGE is greater than 95%.
Synonyms
Resistin-like alpha, RELMalpha, Cysteine-rich secreted protein FIZZ1, Parasite-induced macrophage novel gene 1 protein, Cysteine-rich secreted protein A12-gamma, RELM-a.
Source
Escherichia Coli.
Amino Acid Sequence
MKKLLFAIPL VVPFYSHSTM VNTDETIEII VENKVKELLA NPANYPSTVT TLSCTSVKT MNRWASCPAG MTATGCACGF ACGSWEIQSG DTCNCLCLLV DWTTARCCQL SLEDYKDDDD K.

Product Science Overview

Discovery and Significance

RELM-Alpha was first identified as a protein secreted by adipocytes, the cells responsible for storing fat in the body. It is part of a larger family of proteins known as resistin-like molecules, which also includes RELM-Beta and RELM-Gamma. These proteins share structural similarities and are characterized by a conserved cysteine-rich domain.

Structure and Characteristics

The recombinant form of RELM-Alpha, specifically the mouse variant with a His Tag, is produced using recombinant DNA technology. This involves inserting the gene encoding RELM-Alpha into a host organism, such as Escherichia coli, which then produces the protein. The His Tag is a sequence of histidine residues added to the protein to facilitate purification through affinity chromatography.

The recombinant mouse RELM-Alpha protein is typically expressed in Escherichia coli and purified to a high degree of purity, often greater than 97% . The protein is characterized by its full-length sequence and is validated using techniques such as SDS-PAGE .

Biological Functions

RELM-Alpha plays a crucial role in various biological processes:

  • Glucose Metabolism: It is involved in the regulation of glucose levels in the body, influencing insulin sensitivity and glucose uptake.
  • Inflammation: RELM-Alpha has been shown to modulate inflammatory responses, making it a significant player in conditions such as obesity and diabetes.
  • Immune Response: The protein is also implicated in the immune response, particularly in the context of parasitic infections and allergic reactions.
Applications in Research

Due to its involvement in critical physiological processes, RELM-Alpha is a valuable tool in biomedical research. It is used to study metabolic disorders, inflammatory diseases, and immune responses. The recombinant form, with its high purity and specific tagging, allows for precise experimental manipulation and analysis.

Preparation and Storage

The recombinant mouse RELM-Alpha protein is prepared by expressing the protein in Escherichia coli and purifying it using affinity chromatography. The His Tag facilitates this purification process. The protein is typically shipped at 4°C and should be aliquoted and stored at -20°C upon delivery to avoid freeze/thaw cycles .

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