RELM g Mouse, His
RELM-Gamma Mouse Recombinant, His Tag
Cat. No.
BT21063
Source
Escherichia Coli.
Synonyms
Resistin-like gamma, RELMgamma,RELM-γ, RELM-g.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RELM-gamma Mouse Recombinant is a His -Tagged Fusion Protein having a molecular weight of 11 kDa containing 86 amino acid residues of the RELM-gamma Mouse and 16 additional amino acid residues – HisTag (underlined).
Product Specs
Introduction
RELM-gamma, a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats, exhibits repressed mRNA expression in nasal respiratory epithelium of cigarette smoke-exposed rats compared to untreated rats, as revealed by microarray and real-time RT-PCR experiments. Its physiological tissue-specific expression is highest in hematopoietic tissues, indicating a potential cytokine-like role. RELM-gamma mRNA is detectable in bone marrow, spleen, lung, and peripheral blood granulocytes. Notably, promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid show increased proliferation compared to mock-transfected cells and exhibit an altered response to retinoic acid-induced granulocytic differentiation. These findings suggest that RELM-gamma is a secreted molecule with a restricted expression pattern, potentially involved in promyelocytic differentiation.
Description
RELM-gamma Mouse Recombinant, a His-Tagged Fusion Protein with a molecular weight of 11 kDa, consists of 86 amino acid residues of the RELM-gamma Mouse and 16 additional amino acid residues corresponding to the HisTag (underlined).
Physical Appearance
White lyophilized powder, filtered.
Formulation
The product has been filtered (0.4 μm) and lyophilized from a 0.5 mg/ml solution in 0.05M Acetate buffer at pH 4.
Solubility
To prepare a working stock solution of approximately 0.5 mg/ml, add 0.1M Acetate buffer (pH 4) to the lyophilized pellet and allow it to dissolve completely. For conversion to a higher pH value, dilute the solution extensively with the appropriate buffer to a concentration of 10 μg/ml. Please note that the solubility of this antigen is limited at higher concentrations. This product is not sterile. Before using it in cell culture, filter it through an appropriate sterile filter.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freezing and thawing cycles. Reconstituted protein can be stored at 4°C for a limited time and shows no change after two weeks at this temperature. When stored at -20°C, the lyophilized protein remains stable until the expiration date.
Purity
Greater than 95% purity as determined by SDS-PAGE analysis.
Synonyms
Resistin-like gamma, RELMgamma,RELM-γ, RELM-g.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASHMTLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA.
Product Science Overview
Expression and Function
RELM-gamma is secreted by various tissues including peripheral blood granulocytes, bone marrow, spleen, intestine, and lung . It plays a significant role in promoting and regulating promyelocytic differentiation. Additionally, it is involved in regulating nutrient-associated insulin sensitivity in the intestinal tract .
Molecular Characteristics
Recombinant mouse RELM-gamma is a non-glycosylated protein dimer, containing 89 amino acids and having a molecular mass of approximately 9.4 kDa . The His-tagged version of this protein has a molecular weight of 11 kDa and includes 86 amino acid residues of RELM-gamma mouse along with 16 additional amino acid residues from the His Tag .
Research Applications
Stability and Storage
Solubility
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