RELM g Mouse

RELM-Gamma Mouse Recombinant
Cat. No.
BT20996
Source

Escherichia Coli.

Synonyms

Resistin-like gamma, RELMgamma,RELM-γ, RELM-g. 

Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

RELM g Mouse Recombinant produced in E.Coli is a non glycosylated, homodimeric polypeptide chain containing 2 x 89 amino acids and having a total molecular mass of 18.9kDa.

The RELM is purified by proprietary chromatographic techniques.

Product Specs

Introduction
RELM-gamma, a member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats, shows repressed mRNA expression in nasal respiratory epithelium of rats exposed to cigarette smoke. Its expression is highest in hematopoietic tissues, suggesting a cytokine-like role. RELM-gamma mRNA is found in bone marrow, spleen, lung, and peripheral blood granulocytes. Promyelocytic HL60 cells overexpressing RELM-gamma exhibit increased proliferation and altered response to retinoic acid-induced differentiation, indicating a potential role in promyelocytic differentiation.
Description
Recombinant Mouse RELM g, produced in E. coli, is a non-glycosylated homodimer with two 89 amino acid chains, resulting in a molecular weight of 18.9 kDa. The protein is purified using proprietary chromatographic methods.
Physical Appearance
Sterile white lyophilized powder.
Formulation
Lyophilized from a sterile solution containing 0.1% trifluoroacetic acid (TFA).
Solubility
Reconstitute the lyophilized RELM g in sterile 18 megaohm-cm H2O to a concentration of 100 µg/ml. This solution can be further diluted with aqueous solutions.
Stability
Lyophilized RELM g is stable at room temperature for 3 weeks. For long-term storage, store desiccated below -18°C. Reconstituted RELM g should be stored at 4°C for 2-7 days or below -18°C for future use. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid freeze-thaw cycles.
Purity
Greater than 95% purity as determined by SDS-PAGE.
Synonyms

Resistin-like gamma, RELMgamma,RELM-γ, RELM-g. 

Source

Escherichia Coli.

Amino Acid Sequence

MEGTLESIVE KKVKELLANR DDCPSTVTKT FSCTSITASG RLASCPSGMT VTGCACGYGC GSWDIRDGNT CHCQCSTMDW ATARCCQLA.

Product Science Overview

Structure and Characteristics

Recombinant mouse RELM-γ is a non-glycosylated protein dimer, containing 89 amino acids and having a molecular mass of approximately 9.4 kDa . It is produced in E. coli and is available as a sterile filtered white lyophilized (freeze-dried) powder . The protein is stable when lyophilized at -20°C and should be reconstituted with sterile water at 0.1 mg/mL for further use .

Function and Role

RELM-γ plays a significant role in promoting and regulating promyelocytic differentiation, as well as regulating nutrient-associated insulin sensitivity in the intestinal tract . It is primarily expressed in hematopoietic tissues, suggesting a cytokine-like role . Studies have shown that RELM-γ mRNA is detectable in bone marrow, spleen, lung, and peripheral blood granulocytes .

Research and Applications

RELM-γ is used in various research applications, including studies on promyelocytic differentiation and insulin sensitivity. It is also used to investigate its role in inflammatory responses and its potential as a therapeutic target . Recombinant RELM-γ is available for research purposes and is not intended for human, animal, or diagnostic applications .

Handling and Storage

The lyophilized product is very stable at -20°C. Once reconstituted, it should be aliquoted and frozen at -20°C for long-term storage. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage . The reconstituted protein can be stored at 4°C for a limited period and remains stable for up to two weeks .

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