REG4 Human, Sf9
Sf9, Baculovirus cells.
Sterile filtered colorless solution.
Greater than 90% as determined by SDS-PAGE.
Description
REG4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (23-158 a.a.) and fused to a 9 aa His Tag at C-terminus containing a total of 145 amino acids and having a molecular mass of 17kDa.
REG4 shows multiple bands between 13.5-18kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Sf9, Baculovirus cells.
ADPDIIMRPS CAPGWFYHKS NCYGYFRKLR NWSDAELECQ SYGNGAHLAS ILSLKEASTI
AEYISGYQRS QPIWIGLHDP QKRQQWQWID GAMYLYRSWS GKSMGGNKHC AEMSSNNNFL TWSSNECNKR QHFLCKYRPH HHHHH
Product Science Overview
REG4 was initially identified through high-throughput sequence analysis of a cDNA library derived from patients with inflammatory bowel disease . It is located on the long arm of chromosome 1 and consists of six introns and seven exons. The protein encoded by REG4 comprises 158 amino acids, including a signal peptide of 22 amino acids and a conserved calcium-dependent hydrocarbon recognition domain (CRD), which is crucial for its biological function .
REG4 is abnormally expressed in various cancers, including colorectal, gastric, gallbladder, pancreatic, ovarian, prostate, and lung cancers . It is associated with advanced tumor stages, histological differentiation, and metastasis. REG4-positive cancer cells often exhibit resistance to chemoradiotherapy, particularly 5-FU-based chemotherapy . The protein is involved in several aspects of carcinogenesis, such as cell proliferation, apoptosis, cell cycle regulation, invasion, metastasis, and drug resistance .
Due to its significant role in cancer progression, REG4 is considered a potential diagnostic and prognostic biomarker. It may also serve as a therapeutic target for cancer treatment . The protein’s expression is linked to a relatively unfavorable prognosis in cancer patients, making it a critical focus for ongoing cancer research .
The recombinant form of REG4, specifically the human recombinant produced in Sf9 cells, is utilized for various research and therapeutic purposes. This recombinant protein is often tagged with a His-tag to facilitate purification and detection. The production process involves expressing the REG4 gene in Sf9 cells, a type of insect cell line commonly used for recombinant protein production .
Recombinant REG4 is used in research to study its role in cancer and other diseases. It is also explored as a potential therapeutic agent for conditions such as diabetes mellitus, where it may aid in the regeneration of pancreatic beta cells . Additionally, the protein’s involvement in inflammation and immune response makes it a valuable tool for studying these processes in various disease contexts .
