RBBP9 Human

Retinoblastoma Binding Protein 9 (RBBP9) is a protein encoded by the RBBP9 gene in humans. This protein is known for its interaction with the retinoblastoma protein (pRB), which plays a crucial role in regulating the cell cycle and preventing uncontrolled cell proliferation. The recombinant form of this protein is produced through genetic engineering techniques, allowing for its use in various research and therapeutic applications.

The RBBP9 gene is located on chromosome 20 at the band 20p11.23 . The gene encodes a protein that consists of 186 amino acids and has a molecular weight of approximately 21 kDa . The protein contains a serine hydrolase domain, which is essential for its enzymatic activity .

RBBP9 is involved in several cellular processes, primarily through its interaction with the retinoblastoma protein (pRB). The pRB protein is a well-known tumor suppressor that regulates the cell cycle by controlling the transition from the G1 phase to the S phase . RBBP9 binds to pRB, influencing its ability to regulate cell proliferation and differentiation .

One of the key functions of RBBP9 is its role in the regulation of cell population proliferation. It has been shown to affect cell-cycle control and confer transforming ability, which means it can induce changes in cell behavior that may lead to tumorigenesis . Additionally, RBBP9 has hydrolase activity, which allows it to participate in various biochemical reactions within the cell .

Mutations or dysregulation of the RBBP9 gene have been associated with several diseases, including retinoblastoma and certain types of cataracts . Retinoblastoma is a malignant tumor of the retina that primarily affects young children. The interaction between RBBP9 and pRB is critical in understanding the molecular mechanisms underlying this disease .

The recombinant form of RBBP9 is produced using genetic engineering techniques, which involve inserting the RBBP9 gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the protein for research and therapeutic purposes.

In research, recombinant RBBP9 is used to study its interactions with other proteins, its role in cell cycle regulation, and its involvement in tumorigenesis. Understanding these interactions can provide insights into the development of new therapeutic strategies for diseases associated with RBBP9 dysregulation .