RAMP3 Human
Description
Product Specs
Product Science Overview
Receptor Activity-Modifying Protein 3 (RAMP3) is a member of the RAMP family of single-transmembrane-domain proteins. These proteins play a crucial role in the transport and function of certain receptors on the cell surface. RAMP3, in particular, is involved in the modulation of the calcitonin-receptor-like receptor (CRLR), which can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on the presence of specific RAMPs .
RAMP3 is a type I transmembrane protein with an extracellular N terminus and a cytoplasmic C terminus. The human recombinant form of RAMP3 is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 116 amino acids, with a molecular mass of approximately 13 kDa . It is often fused to a 21 amino acid His-tag at the N-terminus to facilitate purification .
RAMP3 is essential for the proper transport of CRLR to the plasma membrane. When RAMP3 is present, CRLR functions as an adrenomedullin receptor. Adrenomedullin is a peptide involved in various physiological processes, including vasodilation and angiogenesis. RAMP3 also plays a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a GPER1-dependent manner .
The recombinant form of RAMP3 is typically produced using bacterial expression systems such as E. coli. The gene encoding RAMP3 is cloned into an expression vector, which is then introduced into the bacterial cells. The bacteria are cultured, and the protein is expressed and subsequently purified using chromatographic techniques. The final product is a sterile, filtered, colorless solution containing RAMP3 protein in a buffer solution .
The recombinant RAMP3 protein is stable when stored at 4°C for short-term use (2-4 weeks). For long-term storage, it is recommended to store the protein at -20°C, with the addition of a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .
RAMP3 has various applications in research, particularly in studies related to receptor function and signaling pathways. It is used to investigate the mechanisms of receptor transport and the role of adrenomedullin in physiological and pathological processes. Additionally, RAMP3 is valuable in the development of therapeutic strategies targeting cardiovascular diseases and other conditions involving receptor modulation .
