RAMP1 Human

Receptor Activity-Modifying Protein 1 (RAMP1) is a member of the RAMP family of single-transmembrane-domain proteins. These proteins play a crucial role in the functional expression of certain receptors, particularly the calcitonin gene-related peptide (CGRP) receptors. RAMP1 is essential for the transport and proper functioning of these receptors on the cell surface.

RAMP1 is a type I transmembrane protein with an extracellular N-terminus and a cytoplasmic C-terminus. It is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. The CGRP receptor, which has seven transmembrane domains, can function as either a CGRP receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed .

RAMP1 is required to transport the calcitonin-receptor-like receptor (CRLR) to the plasma membrane. In the presence of RAMP1, CRLR functions as a CGRP receptor. This interaction is crucial for the receptor’s ability to respond to its ligands and mediate downstream signaling pathways .

RAMP1 is not limited to interacting with CRLR. It has been shown to interact with other Class II G protein-coupled receptors (GPCRs), including the VPAC1 vasoactive intestinal polypeptide/pituitary adenylate cyclase-activating peptide receptor, glucagon receptor, and parathyroid hormone receptors. These interactions suggest that RAMP1 plays a broader role in modulating cell signaling through various GPCRs .

Recombinant human RAMP1 is produced using various expression systems, including yeast, E. coli, baculovirus, and mammalian cells. This recombinant protein is used in research to study the functional aspects of RAMP1 and its interactions with different receptors. It is also utilized in drug discovery and development to identify potential therapeutic targets for diseases associated with CGRP and other related receptors .

RAMP1 has been implicated in various physiological and pathological processes. It is highly expressed in the cardiovascular system, suggesting a role in vascular function and regulation. Studies have shown that RAMP1 is a key regulator of angiogenesis in endothelial cells, indicating its potential involvement in vascular diseases . Additionally, RAMP1 has been associated with certain cancers, such as pineal dysgerminoma and malignant pineal area germ cell neoplasm .