RAET1L Human
Sf9, Baculovirus cells.
early transcript 1L, ULBP6, UL16-binding protein 6, RAET1L, retinoic acid early transcript 1L protein, retinoic acid early transcript 1L
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
RAET1L Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 202 amino acids (26-218 a.a) and having a molecular mass of 22.9kDa.
RAET1L is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
The protein RAET1L, which stands for Retinoic Acid Early Transcript 1L, functions as a ligand for the NKG2D receptor when the body is under stress. It anchors to cell membranes through a GPI anchor. When the human cytomegalovirus (HCMV) infects a cell, it increases the expression of RAET1L. However, HCMV also manipulates RAET1L's function. The virus uses its own protein, UL16, to trap RAET1L inside the infected cell, preventing it from reaching the cell surface and interacting with the immune system. Compared to other NKG2D ligands, RAET1L is found in a narrower range of cell lines and human tissues.
This product is a recombinant human RAET1L protein produced in Sf9 insect cells using a baculovirus expression system. It consists of a single polypeptide chain with 202 amino acids (specifically, amino acids 26 to 218) and has a molecular weight of 22.9 kDa. The protein is glycosylated, meaning it has sugar molecules attached. For purification and detection purposes, a 6-amino acid histidine tag (His-tag) has been added to the C-terminus of the protein. The protein is purified using proprietary chromatographic techniques.
The product appears as a clear and colorless solution that has been sterilized by filtration.
The RAET1L protein is supplied in a solution containing 1 mg of protein per milliliter (1mg/ml). The solution also contains 10% glycerol and Phosphate-Buffered Saline (PBS) with a pH of 7.4.
To ensure product stability, store the vial at 4°C if you plan to use it within 2-4 weeks. For longer-term storage, freezing the product at -20°C is recommended. If storing for an extended period, consider adding a carrier protein such as HSA or BSA (0.1%) to protect the protein. It is important to minimize the number of times the product is frozen and thawed to maintain protein integrity.
The purity of the RAET1L protein is greater than 95%, as determined by SDS-PAGE analysis.
The biological activity of this product is determined by its ability to bind to human KLRK1 in a functional ELISA assay (using product code pro-2474).
early transcript 1L, ULBP6, UL16-binding protein 6, RAET1L, retinoic acid early transcript 1L protein, retinoic acid early transcript 1L
Sf9, Baculovirus cells.
ADPRRDDPHS LCYDITVIPK FRPGPRWCAV QGQVDEKTFL HYDCGNKTVT PVSPLGKKLN VTMAWKAQNP VLREVVDILT EQLLDIQLEN YTPKEPLTLQ ARMSCEQKAE GHSSGSWQFS IDGQTFLLFD SEKRMWTTVH PGARKMKEKW ENDKDVAMSF HYISMGDCIG WLEDFLMGMD STLEPSAGAP LAMSSGHHHH HH
Product Science Overview
The RAET1L gene encodes glycoproteins that contain extracellular alpha-1 and alpha-2 domains but lack the membrane-proximal Ig-like alpha-3 domain . Most RAET1 glycoproteins are anchored to the membrane via glycosylphosphatidylinositol (GPI) linkage . The RAET1L protein binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity .
RAET1L plays a significant role in the immune system. It is involved in the activation of natural killer (NK) cells and certain T-cells, which are crucial for the body’s innate immune response . The protein’s ability to bind to the KLRK1/NKG2D receptor makes it an essential player in the immune surveillance against tumors and virally infected cells .
