PTP4A1 Human
Protein Tyrosine Phosphatase Type IVA Member 1 Human Recombinant
Cat. No.
BT25418
Source
Escherichia Coli.
Synonyms
Protein tyrosine phosphatase type IVA 1, PTP(CAAXI), Protein-tyrosine phosphatase 4a1, Protein-tyrosine phosphatase of regenerating liver 1, PRL-1, PTP4A1, PRL1, PTPCAAX1, HH72, DKFZp779M0721.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
PTP4A1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids (1-170 a.a.) and having a molecular mass of 21.6kDa. The PTP4A1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Protein tyrosine phosphatase type IVA 1 (PTP4A1), involved in cell signaling, regulates various cellular functions. This unique nuclear PTP is upregulated in regenerating liver and mitogen-stimulated cells. PTP4A1 is primarily found in the spleen, bone marrow, thymus, lymph nodes, T lymphocytes, and tonsils. Its overexpression is observed in tumor cell lines.
Description
Recombinant human PTP4A1, expressed in E. coli, is a non-glycosylated polypeptide chain containing 190 amino acids (1-170 a.a.) with an N-terminal 20 amino acid His tag. This protein, with a molecular weight of 21.6 kDa, is purified using proprietary chromatographic techniques.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The PTP4A1 solution (1 mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 90.0% as determined by SDS-PAGE analysis.
Synonyms
Protein tyrosine phosphatase type IVA 1, PTP(CAAXI), Protein-tyrosine phosphatase 4a1, Protein-tyrosine phosphatase of regenerating liver 1, PRL-1, PTP4A1, PRL1, PTPCAAX1, HH72, DKFZp779M0721.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MARMNRPAPV EVTYKNMRFL ITHNPTNATL NKFIEELKKY GVTTIVRVCE ATYDTTLVEK EGIHVLDWPF DDGAPPSNQI VDDWLSLVKI KFREEPGCCI AVHCVAGLGR APVLVALALI EGGMKYEDAV QFIRQKRRGA FNSKQLLYLE KYRPKMRLRF KDSNGHRNNC.
Product Science Overview
Gene and Protein Structure
The PTP4A1 gene is located on chromosome 6 (6q12) in humans . The protein encoded by this gene contains a PTP domain and a characteristic C-terminal prenylation motif . This prenylation motif is responsible for the protein’s association with the plasma membrane, although PTP4A1 is primarily a nuclear protein .
Biological Functions
PTP4A1 plays a regulatory role in several cellular processes, including:
- Cell Proliferation and Migration: PTP4A1 enhances cell proliferation and motility, which are critical for tissue development and regeneration .
- Cell Cycle Progression: It stimulates the progression from the G1 phase to the S phase during mitosis .
- Cancer Development and Metastasis: Overexpression of PTP4A1 has been linked to cancer metastasis, making it a potential target for cancer therapy .
Cellular Localization
Clinical Significance
Due to its role in cell proliferation and migration, PTP4A1 is implicated in cancer development and metastasis. It has been associated with several cancers, including ovarian cancer and spastic paraplegia 9A . Understanding the function and regulation of PTP4A1 could provide insights into potential therapeutic targets for these diseases.
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