PTH (7-84) N15 Human

Parathyroid hormone (PTH), also known as parathormone, is a critical hormone secreted by the parathyroid glands. It plays a vital role in regulating calcium levels in the blood. The hormone is composed of 84 amino acids and is essential for maintaining calcium homeostasis in the body .

The specific fragment of interest, Parathyroid Hormone (7-84) N15 Labeled (Human Recombinant), refers to a segment of the full-length PTH molecule. This fragment consists of amino acids 7 through 84 of the PTH sequence and is labeled with the stable isotope nitrogen-15 (N15). The labeling with N15 is often used in research to study the hormone’s structure and function through techniques such as nuclear magnetic resonance (NMR) spectroscopy .

The recombinant form of PTH (7-84) N15 is produced using Escherichia coli (E. coli) expression systems. This method involves inserting the gene encoding the PTH fragment into E. coli bacteria, which then produce the hormone fragment. The resulting product is a single, non-glycosylated polypeptide chain containing 78 amino acids and has a molecular mass of approximately 8900 Daltons .

The recombinant PTH (7-84) N15 is purified using proprietary chromatographic techniques to ensure high purity, typically greater than 97% as determined by reverse-phase high-performance liquid chromatography (RP-HPLC). The hormone is usually provided as a sterile, lyophilized (freeze-dried) powder, which can be reconstituted in sterile water for experimental use .

In terms of stability, the lyophilized form of PTH (7-84) N15 is stable at room temperature for up to three weeks. However, for long-term storage, it is recommended to keep the hormone desiccated below -18°C. Upon reconstitution, the hormone should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term storage. To prevent degradation, it is advisable to avoid repeated freeze-thaw cycles .

The N15-labeled PTH (7-84) fragment is primarily used in research settings to study the hormone’s interaction with its receptor and its role in calcium regulation. The stable isotope labeling allows researchers to use advanced techniques like NMR spectroscopy to gain insights into the hormone’s structure and dynamics. This information can be crucial for understanding the mechanisms of PTH action and for developing therapeutic interventions for disorders related to calcium metabolism .