Introduction
Prolactin, a lactogenic hormone produced by the adenohypophysis, is primarily known for its role in lactation. However, it also exhibits various other functions in certain species, including reproductive, maternal behavior, fat metabolism, immunomodulation, and osmoregulation. Prolactin displays cytokine-like properties and significantly influences immune regulation. It contributes to lymphoid tissue development, maintains physiological immune function, and modulates diverse T-cell immune responses. Furthermore, prolactin has been linked to cellular proliferation in non-reproductive tissues such as the liver, spleen, and thymus. It stimulates substantial proliferation in aortic smooth muscle cells, enhancing their proliferation induced by PDGF. Prolactin also demonstrates direct mitogenic effects on pancreatic beta cells and cultured astrocytes.
Description
Recombinant Ovine Prolactin Antagonist, produced in E.Coli, is a single, non-glycosylated polypeptide chain consisting of 199 amino acids with a molecular weight of 23kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Ovine Prolactin was lyophilized from a concentrated (1mg/ml) solution containing 0.02%-0.03% NaHCO3.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized Ovine Prolactin Antagonist in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Biological Activity
Ovine Prolactin Antagonist lacks agonistic activity and effectively inhibits the biological activity of Ovine Prolactin and other lactogenic hormones. This inhibitory effect is demonstrated through proliferation assays using Nb2 cells or other relevant cell lines.
Stability
Lyophilized Ovine Prolactin Antagonist remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at temperatures below -18°C. After reconstitution, Ovine Prolactin Antagonist should be stored at 4°C for 2-7 days. For future use, store it below -18°C. To enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is determined to be greater than 99.0% using SDS-PAGE analysis.
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.