Prolactin Ovine Antagonist

Prolactin Ovine Antagonsit Recombinant
Cat. No.
BT96
Source
Escherichia Coli.
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 99.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Prolactin Ovine Antagonist Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23kDa.
Ovine Prolactin Antagonist is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Prolactin, a lactogenic hormone produced by the adenohypophysis, is primarily known for its role in lactation. However, it also exhibits various other functions in certain species, including reproductive, maternal behavior, fat metabolism, immunomodulation, and osmoregulation. Prolactin displays cytokine-like properties and significantly influences immune regulation. It contributes to lymphoid tissue development, maintains physiological immune function, and modulates diverse T-cell immune responses. Furthermore, prolactin has been linked to cellular proliferation in non-reproductive tissues such as the liver, spleen, and thymus. It stimulates substantial proliferation in aortic smooth muscle cells, enhancing their proliferation induced by PDGF. Prolactin also demonstrates direct mitogenic effects on pancreatic beta cells and cultured astrocytes.
Description
Recombinant Ovine Prolactin Antagonist, produced in E.Coli, is a single, non-glycosylated polypeptide chain consisting of 199 amino acids with a molecular weight of 23kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Ovine Prolactin was lyophilized from a concentrated (1mg/ml) solution containing 0.02%-0.03% NaHCO3.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized Ovine Prolactin Antagonist in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Biological Activity
Ovine Prolactin Antagonist lacks agonistic activity and effectively inhibits the biological activity of Ovine Prolactin and other lactogenic hormones. This inhibitory effect is demonstrated through proliferation assays using Nb2 cells or other relevant cell lines.
Stability
Lyophilized Ovine Prolactin Antagonist remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at temperatures below -18°C. After reconstitution, Ovine Prolactin Antagonist should be stored at 4°C for 2-7 days. For future use, store it below -18°C. To enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is determined to be greater than 99.0% using SDS-PAGE analysis.
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.

Product Science Overview

Production and Purification

The production of Prolactin Ovine Antagonist Recombinant involves the insertion of the ovine prolactin gene into E. coli bacteria, which then express the protein. The protein is subsequently purified using proprietary chromatographic techniques to ensure high purity and biological activity .

Biological Properties and Functions

Prolactin Ovine Antagonist Recombinant is designed to act as an antagonist to prolactin, meaning it binds to prolactin receptors but does not activate them. This can be useful in research and therapeutic contexts where inhibition of prolactin activity is desired. The antagonist form of prolactin can help in studying the hormone’s role in various physiological processes and in developing treatments for conditions where prolactin’s activity needs to be modulated .

Applications in Research and Medicine

The recombinant form of prolactin, including its antagonist, is widely used in laboratory research to study prolactin’s effects on cell proliferation, differentiation, and other cellular processes. It is also used in the development of therapeutic strategies for conditions such as prolactinomas (prolactin-secreting tumors), breast cancer, and other disorders related to prolactin dysregulation .

Stability and Storage

Lyophilized (freeze-dried) Prolactin Ovine Antagonist Recombinant is stable at room temperature for up to three weeks, but for long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. It is recommended to add a carrier protein (such as 0.1% HSA or BSA) to prevent freeze-thaw cycles .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2024 Thebiotek. All Rights Reserved.