ProInsulin Human

Proinsulin is a single-chain polypeptide with a molecular weight ranging from 8 kDa to 10 kDa . It has minimal hormonal activity and is converted into active insulin through the removal of the C-peptide . The mature insulin molecule has 35 fewer amino acids compared to proinsulin, with 4 amino acids removed entirely and the remaining 31 forming the C-peptide .

The ProInsulin C-Peptide Analogue (Human Recombinant) is a synthetic version of the natural C-peptide, produced using recombinant DNA technology. This analogue is a non-glycosylated polypeptide chain containing 35 amino acids and has a molecular mass of approximately 3.6 kDa . It is typically produced in Escherichia coli (E. coli) and purified using proprietary chromatographic techniques .

The recombinant ProInsulin C-Peptide Analogue is used in various research applications, including studies on insulin synthesis, secretion, and function. It is also utilized in the investigation of diabetes and related metabolic disorders. The analogue is lyophilized (freeze-dried) for stability and can be reconstituted with sterile distilled water or an aqueous buffer for use in experiments .

For optimal stability, the lyophilized preparation should be stored at -20°C to -70°C. Upon reconstitution, it can be stored at 2°C to 8°C for up to one week, and for long-term storage, it should be kept at -20°C to -80°C . It is important to avoid repeated freeze-thaw cycles to maintain the integrity of the protein.

The ProInsulin C-Peptide Analogue is a valuable tool in the field of diabetes research. By studying this analogue, researchers can gain insights into the mechanisms of insulin production and secretion, as well as the role of the C-peptide in these processes. This knowledge can contribute to the development of new treatments and therapies for diabetes and other metabolic disorders.