Insulin Human, His
Insulin, Insulin-Dependent Diabetes Mellitus 2, Preproinsulin, Proinsulin, MODY10, IDDM1, IDDM2, IDDM, ILPR, IRDN.
Greater than 95.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
Insulin Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids (25-110 a.a) and having a molecular mass of 11.8kDa. Insulin is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Insulin, Insulin-Dependent Diabetes Mellitus 2, Preproinsulin, Proinsulin, MODY10, IDDM1, IDDM2, IDDM, ILPR, IRDN.
MGSSHHHHHH SSGLVPRGSH MGSFVNQHLC GSHLVEALYL VCGERGFFYT PKTRREAEDL QVGQVELGGG PGAGSLQPLA LEGSLQKRGI VEQCCTSICS LYQLENYCN
Product Science Overview
Insulin is a crucial hormone produced by the pancreas that regulates glucose levels in the blood. It facilitates the uptake of glucose into cells, thereby providing them with energy. Insulin deficiency or resistance leads to diabetes, a condition that affects millions worldwide.
Recombinant human insulin is a form of insulin that is produced using recombinant DNA technology. This method involves inserting the human insulin gene into bacteria or yeast, which then produce insulin that can be harvested and purified. This technology has revolutionized diabetes treatment by providing a consistent and reliable source of insulin.
His Tag (Histidine Tag) is a sequence of histidine residues added to proteins to facilitate their purification. The His tag binds to nickel ions, allowing the tagged protein to be separated from other cellular components using a technique called affinity chromatography.
- Gene Insertion: The human insulin gene is inserted into a plasmid, a small circular piece of DNA.
- Transformation: The plasmid is introduced into a host organism, typically Escherichia coli (E. coli) or yeast.
- Expression: The host organism expresses the insulin gene, producing insulin protein with a His tag.
- Purification: The His-tagged insulin is purified using affinity chromatography, where it binds to a nickel column and is separated from other proteins.
- Refolding: The purified insulin is refolded into its active form and further purified to ensure high quality and activity.
- Diabetes Treatment: Recombinant human insulin is used to treat diabetes, providing a reliable and consistent source of insulin for patients.
- Research: His-tagged insulin is used in research to study insulin’s structure, function, and interactions with other molecules.
- Biotechnology: The His tag facilitates the purification of insulin, making the production process more efficient and cost-effective.
