PGRN Human
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by SDS-PAGE.
Description
Progranulin Human Recombinant produced in HEK is a single, glycosylated, polypeptide chain containing 1-593 amino acids and having a molecular mass of 74kDa. The Progranulin is purified by standard chromatographic techniques.
Product Specs
Recombinant Human Progranulin, produced in HEK cells, is a single, glycosylated polypeptide chain. It consists of 1-593 amino acids and has a molecular weight of 74 kDa. The purification of Progranulin is achieved through standard chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
The protein is supplied in a solution containing 1xPBS (phosphate-buffered saline).
To reconstitute the lyophilized Progranulin, it is recommended to dissolve it in sterile 18MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can then be further diluted as needed using other aqueous solutions.
Lyophilized Progranulin remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, PGRN should be stored at 4°C for 2-7 days. For extended storage, it should be kept at -18°C. Adding a carrier protein, such as 0.1% HSA (human serum albumin) or BSA (bovine serum albumin), is advisable for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein stability.
The purity of the protein is determined to be greater than 98.0% using SDS-PAGE analysis.
The biological activity of Progranulin is demonstrated by its ability to activate phospho-ERK1/2 in neuronal mouse P19 cells. Additionally, it has been shown to play a regulatory role in food intake and body weight.
Product Science Overview
Progranulin is composed of 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif . The precursor protein, progranulin, is cleaved to produce mature granulin peptides, which can be further processed into smaller active peptides named granulin A, B, C, etc. These peptides, along with the intact granulin protein, play crucial roles in regulating cell growth .
Progranulin is involved in a wide range of biological functions:
- Development: It plays a critical role in early embryogenesis and organ development .
- Cell Cycle Progression: Progranulin influences cell proliferation and survival, making it essential for tissue growth and repair .
- Wound Repair: It promotes tissue regeneration and healing by enhancing cell motility and proliferation .
- Inflammation: Progranulin has anti-inflammatory properties and is involved in modulating immune responses .
Progranulin’s role in various diseases has been extensively studied. It is implicated in neurodegenerative diseases such as frontotemporal dementia (FTD) and Alzheimer’s disease. Mutations in the progranulin gene (GRN) can lead to reduced levels of progranulin, contributing to the pathogenesis of these conditions . Additionally, progranulin is involved in cancer biology, where it can act as a growth factor promoting tumorigenesis .
